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Laboratory cultivation of haminoea solitaria and elysia chlorotica



Laboratory cultivation of haminoea solitaria and elysia chlorotica



Veliger 21(2): 299-304



Methods were developed for cultivation of a number of opisthobranch mollusk species currently used as animal models for the study of simple nervous systems. As part of a study on neural and behavioral development in opisthobranchs 2 estuarine opisthobranchis H. solitaria (Order: Cephalaspidea), and E. chlorotica (Order: Sacoglossa) were reared through metamorphosis. Rearing methods were described. H. solitaria had a veliger stage lasting at least 20 days at 18.degree.-24.degree. C. Shell length at hatching averaged 151 .mu.m. Veligers metamorphozed at a shell length of 217 + .mu.m on a primary film. The E. chlorotica veliger measured 132 .mu.m in shell length and settled after at least 16 days at 18.degree.-24.degree. C, at a shell length of 212 + .mu.m. E. chlorotica metamorphozed rarely in response to a primary film. Metamorphosis was induced in this species by a 0.1% solution of succinylcholine chloride in sea water. The natural inducer of metamorphosis in E. chlorotics was unknown.

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