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Laboratory culture and morphology of a marine centric diatom ditylum brightwellii general morphology and vegetative cell division



Laboratory culture and morphology of a marine centric diatom ditylum brightwellii general morphology and vegetative cell division



Japanese Journal of Phycology 30(3): 225-233



Fight and electron microscopy were used. Specimens were collected in Tokyo Bay in 1978 and in Mikawa Bay [Japan] in 1979, respectively, and unialgal cultures were established by the capillary pipette method. The culture was maintained in the room at .apprx. 16.degree. C with a photoperiod of 14 h light and 10 h dark. Cells are prism-shaped, with long centrally located spines, on each valve face. Many delicate spines are also distributed in a row on the edges of valve faces with a density of 4-5/10 .mu.m. Most parts of the cell are occupied by a large vacuole and, due to it, certain cell organelles, such as chloroplasts and mitochondria, are localized in the peripheral regions, except the nucleus which is located almost in the center, being connected to the peripheral regions with radially developed strands of cytoplasm. The nucleus contains 1 nucleolus and is surrounded by many Golgi bodies. Cell divisions usually occurred at the beginning of the dark period, taking 15-30 min to complete the bipartition of cytoplasm.

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