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Laboratory culture of the pelagic blue green alga trichodesmium thiebautii conditions for uni algal culture



Laboratory culture of the pelagic blue green alga trichodesmium thiebautii conditions for uni algal culture



Marine Ecology Progress Series 7(2): 185-190



Conditions for laboratory culture of the pelagic blue-green alga T. thiebautii were investigated and supplementary experiments with T. erythraeum were conducted. T. thiebautii grew in the f medium of Guillard and Ryther after careful removal of living contaminants, especially larvae of the copepod Macrosetella gracilis, from bundle colonies under the microscope. Spherical colonies were not suitable for the inoculum. T. thiebautii appears to be auxotrophic, at least in terms of B12 requirement, under our culture conditions. T. erythraeum survived up to 100 days in f medium but failed to grow actively.

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