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Laboratory diagnosis of viral diseases of domestic animals at universiti pertanian malaysia by electron microscopy



Laboratory diagnosis of viral diseases of domestic animals at universiti pertanian malaysia by electron microscopy



Pertanika 4(2): 137-140



Clinical and postmortem specimens in the form of warts, scabs, feces, trachea and lungs from suspected cases of bovine papilloma, contagious ecthyma of goat, fowl pox, calf diarrhea and avian infectious bronchitis were submitted for laboratory diagnosis. Examination by EM revealed coronavirus-like particles in the feces of a calf with diarrhea and in the allantoic fluid of embryonated eggs infected with materials from a fowl suspected of infectious bronchitis. Poxvirus-like particles were observed from the scab of a goat with clinical symptoms of contagious ecthyma and also from the scab of a chicken suspected of fowl pox. Parvovirus-like particles were detected from the feces of a dog with diarrhea. Examination of warts from 2 different cattle, both clinically diagnosed as bovine papilloma, revealed different results: in 1 case, only papilloma virus was present; in the other, both papillomavirus and poxvirus-like particles were present.

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