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Laboratory equipment for the carbon 11 carbon dioxide and carbon 14 carbon dioxide labeling of plants and for in vivo detection of the carbon 11 labeled assimilate flow into the root

Laboratory equipment for the carbon 11 carbon dioxide and carbon 14 carbon dioxide labeling of plants and for in vivo detection of the carbon 11 labeled assimilate flow into the root

Angewandte Botanik 60(3-4): 187-200

Equipment for measuring the photosynthesis rate, for leaf application with 11CO2 or alternatively 14CO2, as well as for detecting the 11C-labelled assimilate transport into the root is presented and discussed. The gas distribution equipment, an exchangeable leaf cuvette connected to a gas circulation system, is a semi-closed system in which moisture content, temperature and CO2 content of the air can be controlled. An CO2 generator enables long-time labelling to be carried out with 14CO2 for balancing experiments and metabolic studies. Pulse labelling with the short-lived carbon isotope 11C permits non-destructive in vivo measurements of the assimilate transport using externally attached detectors. For soybeans short-time kinetics on assimilate-root transport under defined conditions are possible by means of an appropriate lead shielding with a stalk penetration and by focusing the detector onto a standardized flow-root cuvette.

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