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Laboratory evaluation of commercial formulations of bacillus thuringiensis var israelensis against mosquito aedes aegypti and black fly simulium spp larvae



Laboratory evaluation of commercial formulations of bacillus thuringiensis var israelensis against mosquito aedes aegypti and black fly simulium spp larvae



Journal of Agricultural Entomology 1(2): 161-168



Three commercial black fly and mosquito larvicidal formulations of B. thuringiensis Berliner var. israelensis de Barjac were bioassayed to determine their potencies relative to the international standard powder, IPS-78. The potencies of the wettable powder (WP) formulations of Bactimos and Vectobac and the water dispersible concentrate (WDC) Teknar were determined to be 4530, 5723, and 336 International Toxic Units (ITU)/mg, respectively, against Simulium spp. [S. vittatum, S. verecundum]; their respective ITU/mg values against A. aegypti (Linnaeus) were 3556, 2317, and 1373. Little correlation (r2 = 0.47) was evident between the potency rating (ITU/mg) of the formulations against mosquitoes and black flies; this indicated that the potency rating of a formulation against black flies cannot be simply predicted from the A. aegypti ITU/mg rating on its label. A moderate correlation between formulation potency and mean particle size was observed in the black fly assays (r2 = 0.77), but not against mosquitoes (r2 = 0.20). Aqueous suspensions of Teknar-WDC had a significantly smaller mean particle size and stayed significantly longer in suspension than other formulations. The relatively long duration of suspension of Teknar-WDC was viewed as important since the toxic crystals would be less likely to settle out in mosquito and black fly habitats. A ratio based on the LC50 values determined from standardized bioassay methods is suggested as a convenient way to express the relative potency of a formulation against mosquitoes and black flies.

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