+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Laboratory evaluation of the serum dilution test in serious staphylococcal infection

Laboratory evaluation of the serum dilution test in serious staphylococcal infection

American Journal of Clinical Pathology 80(2): 176-181

Four test media were studied to determine performance characteristics of serum dilution tests used to monitor antimicrobial therapy during serious Staphylococcus aureus infection being treated with highly protein-bound antibiotics. Serum inhibitory titers and serum bactericidal titers obtained with Mueller-Hinton broth supplemented with calcium and magnesium were 3- to 16-fold higher than titers obtained with whole human serum buffered with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES). In cation-supplemented Mueller-Hinton containing 5% albumin or in cation-supplemented Mueller-Hinton combined with an equal volume of human serum, titers were 2- to 5-fold higher than in whole human serum buffered with HEPES. Clinical or animal studies are needed to establish whether the higher titers observed with patient serum containing highly protein-bound drugs diluted in low protein-content media would foster inadequate dosage regiments. In the meantime, both infectious disease clinicians and microbiologists should be aware of this potential pitfall.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005787855

Download citation: RISBibTeXText

PMID: 6554031

DOI: 10.1093/ajcp/80.2.176

Related references

Paracoagulation tests in laboratory verification of intravascular coagulation. Comparative clinical evaluation of the SDPS test and of the ethanol test with serial dilution (SD ethanol test). Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 11(1): 159-175, 1975

Evaluation of the efficacy of laboratory diagnosis of typhoid infection. (Latex test, conditioned hemagglutination, adenosine deaminase activity in the serum). Giornale di Malattie Infettive E Parassitarie 24(4): 296-299, 1972

Evaluation of the diagnostic significance of the modified hemolysis inhibition test in staphylococcal infection. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (8): 53-58, 1980

Development and evaluation of a laboratory information system-based auto-dilution and manual dilution algorithm for alpha-fetoprotein assay. Annals of Laboratory Medicine 33(5): 390-392, 2014

Studies on staphylococcal infection. I. Serum anticoagulase titer in experimental staphylococcal pneumonia and subcutaneous abscess in rabbits. Sapporo Igaku Zasshi. Sapporo Medical Journal 26(6): 266-270, 1964

Studies on staphylococcal infection. II. Serum anticoagulase titer in healthy human subjects and the patients suffering from the various staphylococcal infections. Sapporo Igaku Zasshi. Sapporo Medical Journal 26(6): 271-279, 1964

The applicability of the rosette dilution test for the determination of haptoglobin concentration--a simple test for the clinical hematology laboratory. Blut 16(1): 12-16, 1967

Evaluation of the E-test: comparison of E-test and microbroth dilution method in azole antifungal susceptibility test. Nihon Ishinkin Gakkai Zasshi 39(1): 27-31, 1998

Laboratory evaluation of the specific serum antimycobacterial activity test. M: Nd, And G. M. Savage, Editors. Antimicrobial Agents And Chemotherapy 1961. Proceedings Of A Conference. 20-29, 1962

The evaluation of the serum cholinesterase activity as a laboratory test from the biostatistic viewpoint. Das Deutsche Gesundheitswesen 16: 1019-1022, 1961

Laboratory evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection. Journal of Clinical Microbiology 53(1): 311-313, 2015

An evaluation of the venereal disease research laboratory test and fluorescent treponema antibody absorption test in a general hospital laboratory. American Journal Of Clinical Pathology: 420-424, 1969

Staphylococcal infection of the tail of the laboratory rat. Laboratory Animal Care 16(5): 421-429, 1966

Laboratory diagnosis. (11). Problems in the urinary concentration-dilution test. Saishin Igaku. Modern Medicine 21(5): 1081-1095, 1966

Use of Serum Ultrafiltrate in the Serum Dilution Test. Journal of Infectious Diseases 160(4): 616-623, 1989