+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Laboratory investigation of diarrhea in travelers to mexico evaluation of methods for detecting entero toxigenic escherichia coli



Laboratory investigation of diarrhea in travelers to mexico evaluation of methods for detecting entero toxigenic escherichia coli



Journal of Clinical Microbiology 3(5): 486-495



A laboratory investigation was conducted on cultures collected from travelers before, during and after a trip to Mexico to characterize the etiology of traveler's diarrhea. Four laboratory methods for detecting enterotoxigenicity of E. coli were evaluated: the infant mouse assay, the Chinese hamster ovary (CHO) cell assay, the Y1 [mouse] adrenal [tumor] cell assay and the rabbit ileal loop. Although a number of common enteric pathogens were identified as a cause of traveler's diarrhea, including 6 serotypes of Salmonella, 2 serotypes of Shigella, Vibrio parahaemolyticus, Giardia lamblia and Entamoeba histolytica, enterotoxigenic E. coli was most commonly isolated. Strains were identified that produced only heat-labile enterotoxin (LT), only heat-stable enterotoxin (ST), or both LT and ST. The infant mouse assay yielded results falling into 2 distinct groups, providing a clear separation of positive and negative cultures. The CHO assay also formed 2 groups, with positive cultures producing 11% or more of the elongated cells. There was good agreement between the CHO and the Y1 adrenal cell assays for detection of LT. The adrenal cell system for detection of LT was more suitable than the CHO assay for processing large numbers of specimens because of the miniculture modification of this method utilized. The infant mouse method was a simple and reliable method for detecting ST.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005787973

Download citation: RISBibTeXText

PMID: 180047


Related references

Relationship between entero toxigenic escherichia coli virulence factors and travelers diarrhea. Nelson, J D And C Grassi (Ed ) Current Chemotherapy And Infectious Disease, Vol 1 And 2; Proceedings Of The 11th International Congress And The 19th Interscience Conference on Antimicrobial Agents And Chemotherapy, Boston, Mass , Usa, Oct 1-5, 1979 Xxi+774p (Vol 1); Ix+1023p (Vol 2 ) The American Society For Microbiology: Washington, D C , Usa Illus P917-919, 1980

Serological evidence for the role of entero toxigenic escherichia coli in travelers diarrhea. Abstracts of the Annual Meeting of the American Society for Microbiology 78 : 279, 1978

Serological evidence for the role of colonization factor antigens in entero toxigenic escherichia coli diarrhea of travelers. Abstracts of the Annual Meeting of the American Society for Microbiology 78 : 279, 1978

Mannose resistant hem agglutinins on entero toxigenic escherichia coli of serotype o 6 k 15 h 16 or h minus isolated from travelers and infantile diarrhea. Journal of General Microbiology 128(9): 2081-2096, 1982

Colonization of the upper jejunum by entero pathogenic and entero toxigenic escherichia coli in pediatric diarrhea. Acta Paediatrica Scandinavica 71(3): 457-466, 1982

Frequency of the isolation of entero pathogenic and entero toxigenic escherichia coli from adult diarrhea patients. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (2): 70-73, 1982

Identification of entero toxigenic escherichia coli in patients with diarrhea in asia with 3 entero toxin gene probes. Infection & Immunity 42(1): 152-155, 1983

Travelers' diarrhea and toxigenic Escherichia coli. New England Journal of Medicine 292(18): 933-936, 1975

Pathogenic effect of entero toxigenic escherichia coli and escherichia coli causing infantile diarrhea. Acta Microbiologica Academiae Scientiarum Hungaricae 24(3): 221-236, 1977

Heat labile entero toxigenic escherichia coli and intestinal protozoa in asymptomatic travelers. Southeast Asian Journal of Tropical Medicine and Public Health 8(4): 476-479, 1977

Epidemiology of cholera and entero toxigenic escherichia coli diarrhea. Ouchterlony, O And J Holmgren (Ed ) Nobel Symposium, Vol 43 Cholera And Related Diarrheas: Molecular Aspects Of A Global Health Problem; Stockholm, Sweden, Aug 6-11, 1978 Xi+251p S Karger: Basel, Switzerland; New York, N Y , Usa Illus P34-45, 1980

A new diarrhea pathogen: entero-adherent-invasive-toxigenic Escherichia coli. Chinese Medical Journal 109(1): 16-17, 1996

Occurrence of entero toxigenic escherichia coli in calves with acute neo natal diarrhea. Nordisk Veterinaermedicin 35(10): 346-352, 1983

Entero toxigenic escherichia coli in fecal samples from acute infant diarrhea. Igiene Moderna 75(2): 213-222, 1981

Several serotypes of entero toxigenic escherichia coli and reovirus like agents in pediatric diarrhea. Pediatric Research 12(4 PART 2): 491, 1978