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Laboratory investigations on neuro paralytic accidents associated with suckling mouse brain rabies vaccine part 3 preservation of vaccine potency after elimination of murine brain myelin by centrifugation



Laboratory investigations on neuro paralytic accidents associated with suckling mouse brain rabies vaccine part 3 preservation of vaccine potency after elimination of murine brain myelin by centrifugation



Annales de Microbiologie (Paris) 127B(4): 567-572



Myelin found in 9 day-old mouse brain was eliminated from weanling mouse brain suspensions by centrifugation at 17,000 g for 10 min, as demonstrated by EM and guinea pig inoculation tests for encephalitogenic activity. This centrifugation procedure did not affect the potency of 7 batches of suckling mouse brain vaccine, when centrifuged and non-centrifuged samples of the same batches were compared by a modified NIH potency test (t = 0.17). These results suggest that centrifugation at 17,000 g be used in the preparation of rabies suckling mouse brain vaccine instead of the 1900 g currently employed, which does not eliminate myelin. This new procedure would probably reduce the number of post-vaccine reactions which are attributable to the small amount of myelin which remains in vaccines prepared with new-born animal brains following the current procedure.

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Related references

Laboratory investigations on neuroparalytic accidents associated with suckling mouse brain rabies vaccine. III. -- Preservation of vaccine potency after elimination of murine brain myelin by centrifugation. Annales de Microbiologie 127b(4): 567-572, 1976

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