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Laboratory tests of the novosibirskii strain of the nuclear polyhedrosis virus on the nun moth lymantria monacha 4 5 instar larvae



Laboratory tests of the novosibirskii strain of the nuclear polyhedrosis virus on the nun moth lymantria monacha 4 5 instar larvae



Entomologicheskoe Obozrenie 58(2): 421-425



The effect of various concentrations of nuclear polyhedrosis virus (Novosibirskii strain) on 4.sbd.5 instar larvae of the nun moth was studied. Suspensions with titers of 108, 107, 8, 3 .times. 106, 9, 0 .times. 106 and 7.6 .times. 106 polyhedra in 1 cm3 were prepared. Best results were obtained with a concentration of 108 polyhedra/cm3. A lethal concentration [LC50] was experimentally determined for 4.sbd.5 instar larvae which was equal to 0.158 .times. 108. Using the line of regression not only LC84% = 0.447 .times. 1010 but also LC90% but also LC90% and LC100% could be determined. Allowance should be made for the difference between destruction percentage under natural conditions and the theoretical percentage obtained by using a line of regression. The incubation period of the disease was 6 days. Mortality of individuals in the pupal stage by the nuclear polyhedrosis virus was also determined.

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Laboratory tests of nucleopolyhedrosis virus Lymantria monacha L. Novosibirsk strain on the 4-5 instar caterpillars of the nun moth. Entomologicheskoe Obozrenie 582: 421-425, 1979

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