Lectin affinity electrophoresis of human alpha fetoprotein detection of separated bands by antibody affinity blotting and its clinical use

Taketa, K.

Japanese Journal of Electrophoresis 30(1): 3-10


Accession: 005800266

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A sensitive technique of antibody-affinity blotting was developed for the detection of .alpha.-fetoprotein(AFP) bands separated by affinity electrophoresis with concanavalin A(Con -A), Lens culinaris agglutinin A(LCA-A) and erythroagglutinating phytohemagglutinin(E-PHA). Nitrocellulose membranes precoated with antibodies to human AFP were used for the blotting and the transferred AFP bands were visualized by an immuno-enzymatic method. As low as 4 pg/mm2 AFP was detected, and application of 4 .mu.l of 200 ng/ml AFP allowed us to quantitate two to five separated bands. Con-A-nonreactive AFP, LCA-A-reactive AFP and E-PHA-reactive AFP increased in hepatocellular carcinoma and other extrahepatic tumors but not in hepatitis and liver cirrhosis. Combined evaluation of these results showed diagnostic values of 89-95% in sensitivity, depending on the combination, with high specificities above 92%.