Leukocyte associated plasma proteins association of prealbumin albumin oroso mucoid alpha 1 anti trypsin transferrin and hapto globin with human lymphocytes monocytes granulocytes and a pro myelocytic leukemic cell line hl 60

Andersen, M.M.

Scandinavian Journal of Clinical and Laboratory Investigation 43(1): 49-60


ISSN/ISBN: 0036-5513
Accession: 005804788

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The distribution of prealbumin, albumin, orosomucoid, .alpha.1-antitrypsin, haptoglobin and transferrin, including their electrophoretic heterogeneous variants, was studied in isolated lymphocytes, monocytes and granulocytes and in a human promyelocytic cell line (HL-60) by crossed immunoelectrophoresis. Prealbumin, albumin and transferrin were present in lymphocytes, monocytes and granulocytes, the cellular variants of orosomucoid and haptoglobin were present only in granulocytes. .alpha.1-Antitrypsin was present in 4 electrophoretic variants which were differently distributed among the various cell types. Synthesis of .alpha.1-antitrypsin by monocytes, granulocytes and HL-60 cells was demonstrated by 14C-leucine incorporation. The 6 plasma proteins could not be removed from intact cells by incubation with the respective antibodies at 0.degree. C or iodinated by lactoperoxidase catalyzed iodination at 23.degree. C. They were readily solubilized by freeze-lysis of the cells, suggesting an intracellular localization. Compared to their plasma counterparts none of the proteins differed in their hydrophobic properties, but the carbohydrate residues of orosomucoid, .alpha.1-antitrypsin and haptoglobin were different. The pattern of disappearance of the proteins from the cells during incubation suggested that the localization of albumin and transferrin in relation to the cells differed from that of the other proteins.