Light dependent rubidium uptake into isolated mesophyll protoplasts from leaves of pisum sativum cultivar cebeco 204 primo

Leurs, C.J.; Winter, H.; Wiersema, P.K.; Helder, R.J.

Physiologia Plantarum 56(3): 339-342

1982


ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1982.tb00349.x
Accession: 005809818

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Abstract
A method is described for the isolation of large amounts of physiologically active protoplasts from leaves of P. sativum L. Rb uptake was determined after separation of the intact protoplasts from the loading medium by rapid centrifugation through a phthalate step gradient. In freshly isolated mesophyll protoplasts of P. sativum, Rb uptake was carbonylcyanide-p-trifluoromethoxyphenylhydrazone reduced by metabolic inhibitors such as 5 .mu.M, 0.1 mM cyanide, 2 .mu.M DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea] and 5 mM arsenate and by dark incubation. Reduction of Rb uptake by inhibition of aerobic respiration or the photosynthetic electron transport system demonstrates that both processes play a role in the energy supply for membrane transport in these protoplasts.

Light dependent rubidium uptake into isolated mesophyll protoplasts from leaves of pisum sativum cultivar cebeco 204 primo