Section 6
Chapter 5,814

Link protein and a hyaluronic acid-binding region as components of aorta proteoglycan

Gardell, S.; Baker, J.; Caterson, B.; Heinegård, D.; Rodén, L.

Biochemical and Biophysical Research Communications 95(4): 1823-1831


ISSN/ISBN: 0006-291X
PMID: 7417346
DOI: 10.1016/s0006-291x(80)80111-2
Accession: 005813495

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Proteoglycans from bovine aorta were extracted with 4 M guanidine HCl in the presence of protease inhibitors and fractionated by density gradient centrifugation in CsCl. The bottom fraction from a dissociative gradient contained proteoglycans with a size distribution similar to that of cartilage proteoglycans. Antigens were present which reacted with antisera raised against the hyaluronic acid-binding region and chondroitin sulfate peptides from cartilage proteoglycans. By associative and dissociative density gradient centrifugations, a fraction was obtained which was precipitated by antiserum to link proteins of bovine nasal cartilage and had the same electrophoretic mobility as link protein 1.

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