Link protein and a hyaluronic acid-binding region as components of aorta proteoglycan
Gardell, S.; Baker, J.; Caterson, B.; Heinegård, D.; Rodén, L.
Biochemical and Biophysical Research Communications 95(4): 1823-1831
ISSN/ISBN: 0006-291X PMID: 7417346 DOI: 10.1016/s0006-291x(80)80111-2
Proteoglycans from bovine aorta were extracted with 4 M guanidine HCl in the presence of protease inhibitors and fractionated by density gradient centrifugation in CsCl. The bottom fraction from a dissociative gradient contained proteoglycans with a size distribution similar to that of cartilage proteoglycans. Antigens were present which reacted with antisera raised against the hyaluronic acid-binding region and chondroitin sulfate peptides from cartilage proteoglycans. By associative and dissociative density gradient centrifugations, a fraction was obtained which was precipitated by antiserum to link proteins of bovine nasal cartilage and had the same electrophoretic mobility as link protein 1.