Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography
Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography
Haddad, A.; Kopriwa, B.; Pelletier, G.
Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society 35(10): 1059-1062
1987
ISSN/ISBN: 0022-1554
PMID: 3305700
DOI: 10.1177/35.10.3305700
To determine whether or not the secretory granules of insulin-secreting cells contained glycoproteins, isolated rat pancreatic islets were incubated for 2 and 4 hr in a medium containing L-[3H]-fucose. Quantitative analysis of high-resolution electron microscopic autoradiographs of the insulin-secreting beta cells demonstrated that glycoproteins with fucose residues are contained within the insulin secretory granule.