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Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography

Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography

Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society 35(10): 1059-1062

ISSN/ISBN: 0022-1554

PMID: 3305700

DOI: 10.1177/35.10.3305700

To determine whether or not the secretory granules of insulin-secreting cells contained glycoproteins, isolated rat pancreatic islets were incubated for 2 and 4 hr in a medium containing L-[3H]-fucose. Quantitative analysis of high-resolution electron microscopic autoradiographs of the insulin-secreting beta cells demonstrated that glycoproteins with fucose residues are contained within the insulin secretory granule.

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Accession: 005823491

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