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Lymphocyte transformation test with spermatozoal antigens in men from infertile couples part 1 a methodological study of different spermatozoal preparations as antigen

Lymphocyte transformation test with spermatozoal antigens in men from infertile couples part 1 a methodological study of different spermatozoal preparations as antigen

International Journal of Fertility 21(4): 218-225

ISSN/ISBN: 0020-725x

The reactivity of lymphocytes from 63 men from infertile couples was investigated in a lymphocyte-transformation test (LTT) after application of different spermatozoal antigens. As antigens washed spermatozoa were used, autologous or allogenic, in the latter case either from a single person or pooled spermatozoa from different persons. Concentrations of 3 .times. 105 and 15 .times. 105 spermatozoa per ml of culture were used since higher concentrations could cause suppression of the reactivity of the lymphocytes. After stimulation with autologous spermatozoa, 14% showed reactivity at least 3 times as high as in the control cultures. After use of pooled spermatozoa and allogenic spermatozoa from a single person, a similar reactivity was found in 11 and 24%, respectively. There was a fairly close correlation between the results obtained in the same person after stimulation with autologous spermatozoa and pooled spermatozoa, but the responses to allogenic spermatozoa tended to be higher than those seen after addition of autologous spermatozoa. This higher frequency of positive reactions after stimulation with allogenic spermatozoa did not seem to be due to the presence of leukocytes in the sperm sample which was used for the preparation of allogenic antigen, but seemed mainly to depend on the presence of foreign HL-A antigen on the spermatozoa. Coating of spermatozoa with agglutinating auto-antibodies did not influence the results of the LTT.

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Accession: 005839194

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