EurekaMag
+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

Lymphocyte transformation test with spermatozoal antigens in men from infertile couples part 1 a methodological study of different spermatozoal preparations as antigen


Lymphocyte transformation test with spermatozoal antigens in men from infertile couples part 1 a methodological study of different spermatozoal preparations as antigen



International Journal of Fertility 21(4): 218-225



ISSN/ISBN: 0020-725x

The reactivity of lymphocytes from 63 men from infertile couples was investigated in a lymphocyte-transformation test (LTT) after application of different spermatozoal antigens. As antigens washed spermatozoa were used, autologous or allogenic, in the latter case either from a single person or pooled spermatozoa from different persons. Concentrations of 3 .times. 105 and 15 .times. 105 spermatozoa per ml of culture were used since higher concentrations could cause suppression of the reactivity of the lymphocytes. After stimulation with autologous spermatozoa, 14% showed reactivity at least 3 times as high as in the control cultures. After use of pooled spermatozoa and allogenic spermatozoa from a single person, a similar reactivity was found in 11 and 24%, respectively. There was a fairly close correlation between the results obtained in the same person after stimulation with autologous spermatozoa and pooled spermatozoa, but the responses to allogenic spermatozoa tended to be higher than those seen after addition of autologous spermatozoa. This higher frequency of positive reactions after stimulation with allogenic spermatozoa did not seem to be due to the presence of leukocytes in the sperm sample which was used for the preparation of allogenic antigen, but seemed mainly to depend on the presence of foreign HL-A antigen on the spermatozoa. Coating of spermatozoa with agglutinating auto-antibodies did not influence the results of the LTT.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005839194

Download citation: RISBibTeXText

Related references

Lymphocyte transformation test with spermatozoal antigens in men from infertile couples part 2 clinical studies. International Journal of Fertility 21(4): 226-231, 1976

Immunofluorescent studies on human spermatozoa. II. Characterization of spermatozoal antigens and their occurrence in spermatozoa from the male partners of infertile couples. Clinical and Experimental Immunology 9(1): 21-31, 1971

The improved cyto toxicity immobilization test with the aid of multiple exposure photography method for the detection of anti spermatozoal antibodies in sera of infertile couples. Fertility and Sterility 41(2 Suppl): 100S, 1984

Anti-spermatozoal antibodies in infertile couples. Hawaii Medical Journal 35(6): 166-170, 1976

Induction of high titer mouse--antihuman spermatozoal antibodies by liposome incorporation of spermatozoal membrane antigens. American Journal of Reproductive Immunology: Ajri: Official Journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction 4(3): 127-132, 1983

Induction of high titer mouse-antihuman spermatozoal antibodies by lyosome incorporation of spermatozoal membrane antigens. American Journal of Reproductive Immunology 4(3): 127-132, 1983

Spermatozoal density, motility and morphology in fertile and in infertile couples: a mathematical correlation. Acta Europaea Fertilitatis 15(2): 119-122, 1984

Daily spermatozoal production and epididymal spermatozoal reserves of the human male. Journal of Urology 124(2): 211-215, 1980

Interference of human spermatozoal motility and spermatozoal agglutination by Candida albicans. Journal of Urology 118(5): 797-799, 1977

The effects of frequent ejaculation on seminal spermatozoal number and calculation of daily spermatozoal output. Clinical Reproduction and Fertility 3(2): 145-149, 1985

Stallion spermatozoal morphology and its relationship to spermatozoal motility and fertility. Proceedings of the 37th Annual Convention of the American Association of Equine Practitioners, San Francisco, USA, 1-4 December 1991: 211-221, 1991

The spermiogram, spermatozoal reserves and spermatozoal transport in the epididymis of the rabbit.. Nauchni Trud. vissh selskostop. Inst. Georgi Dimitrov zootekh. Fak 13: 135-159, 1963

Immuno fluorescent studies on human spermatozoa part 1 the detection of different spermatozoal antibodies and their occurrence in normal and infertile women. Clinical and Experimental Immunology 8(1): 9-23, 1971

Daily spermatozoal production, epididymal spermatozoal reserves and transit time of spermatozoa through the epididymis of the rhesus monkey. Biology of Reproduction 15(5): 586-592, 1976

Spermatozoal antibodies and fertility I. Attempt to induce temporary sterility in female white mice by passive immunization with spermatozoal antisera. Jour Immunol 38(2): 97-103, 1940