EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Maturation and hatching of eggs from silkworm bombyx mori ovaries preserved in liquid nitrogen


Maturation and hatching of eggs from silkworm bombyx mori ovaries preserved in liquid nitrogen



Journal of Insect Physiology 31(12): 963-967



ISSN/ISBN: 0022-1910

Ovarian imaginal discs prepared from fifth-instar larvae of the silkworm, Bombyx mori were treated with graded concentrations of glycerol, cooled at a rate of 1.degree. C/min to -35.degree. C and preserved in liquid nitrogen for 2 days or more and then rapidly thawed (500.degree. C/min). The frozen and thawed ovaries were transplanted into fifth-instar female larvae, in which more than 20% of the ovaries developed to produce mature eggs with a chorion according to the state of host development. By parthenogenetic activation, the mature eggs started embryogenesis and hatched to produce larvae. About 50% hatching occurred in the eggs developed in a C 108 .times. Cambodge host, and about 10% in a C 108 .times. Aojuku host. The hatched larvae completed post-embryonic development as did the normal larvae.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005858683

Download citation: RISBibTeXText

Related references

Maturation and hatching of eggs from silkworm ovaries preserved in liquid nitrogen. Journal of Insect Physiology 3112: 963-967, 1985

Survival of larval ovaries and testes frozen in liquid nitrogen in the silkworm bombyx mori. Cryobiology 26(4): 389-396, 1989

New ecdysteroids from ovaries and eggs of the silkworm, Bombyx mori. Zoological Science 11(Suppl ): 19, 1994

Studies on the rhythmicity of hatching in silkworm eggs, Bombyx mori L. Jour Fac Textile Sci And Technol Shinshu Univ Ser E Agric And Sericult 5: 69-123, 1961

2-deoxy-a-ecdysone from ovaries and eggs of the silkworm, Bombyx mori. Science, N No.,4298: 66-67, 1977

Free-running rhythm of hatching in the silkworm eggs, Bombyx mori L. New Entomologist Ueda 185: 45-51, 1972

Artificial hatching of silkworm eggs (Bombyx mori L.) after different chilling periods. Indian Journal of Sericulture 36(1): 47-50, 1997

Hatching patterns of black boxed eggs in silkworm, Bombyx mori. Journal of Experimental Zoology, India 4(1): 67-69, 2001

2-Deoxy-alpha-ecdysone from ovaries and eggs of the silkworm, Bombyx mori. Science 197(4298): 66-67, 1977

Determining suitable wintering and hatching regimes of the eggs of the silkworm bombyx mori. Zhivotnov"dni Nauki 5(3): 85-94, 1968

Effect of some biologically active substances on hatching of eggs of silkworm, Bombyx mori L. Journal of Entomological Research 25(1): 47-51, 2001

Artificial hatching of hibernated eggs of silkworm bombyx mori l. by warm water treatment. Bangladesh Journal of Zoology 17(2): 117-122, 1989

The existence of diapause hormone in adult ovaries and mature eggs of the silkworm bombyx mori. Journal of Sericultural Science of Japan 46(3): 239-245, 1977

Free-running rhythm of hatching in the silkworm eggs (Bombyx mori L.) differing in the larval moltinisms. New Ent: 53-60, 1976

Some factors related to hatching ability of long term chilled eggs of the silkworm bombyx mori. Journal of Sericultural Science of Japan 58(5): 387-391, 1989