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Maturation and hatching of eggs from silkworm bombyx mori ovaries preserved in liquid nitrogen

Maturation and hatching of eggs from silkworm bombyx mori ovaries preserved in liquid nitrogen

Journal of Insect Physiology 31(12): 963-967

ISSN/ISBN: 0022-1910

Ovarian imaginal discs prepared from fifth-instar larvae of the silkworm, Bombyx mori were treated with graded concentrations of glycerol, cooled at a rate of C/min to C and preserved in liquid nitrogen for 2 days or more and then rapidly thawed ( C/min). The frozen and thawed ovaries were transplanted into fifth-instar female larvae, in which more than 20% of the ovaries developed to produce mature eggs with a chorion according to the state of host development. By parthenogenetic activation, the mature eggs started embryogenesis and hatched to produce larvae. About 50% hatching occurred in the eggs developed in a C 108 .times. Cambodge host, and about 10% in a C 108 .times. Aojuku host. The hatched larvae completed post-embryonic development as did the normal larvae.

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Accession: 005858683

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