+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Membrane lipid biosynthesis in Acholeplasma laidlawii B: de novo biosynthesis of saturated fatty acids by growing cells



Membrane lipid biosynthesis in Acholeplasma laidlawii B: de novo biosynthesis of saturated fatty acids by growing cells



Journal of Bacteriology 132(2): 497-504



The de novo biosynthesis of fatty acids of 12 to 18 carbons from precursors of 5 carbons or fewer has been demonstrated in Acholeplasma laidlawii B. Radiolabeling experiments indicated that the normal primers for the synthesis of the even- and odd-chain fatty acids are acetate and propionate or valerate, respectively. Saturated straight-chain monomethyl-branched fatty acids of up to five carbons were readily utilized as primers, wheras more highly branched species and those possessing halogen substituents or unsaturation were not utilized. At primer concentrations of 1 to 3 mM, up to 80% of the total cellular lipid fatty acids were derived from exogenous primer. The mean chain length of the exogenous primer-derived fatty acids rose with increasing primer incorporation for methyl-branched short-chain fatty acids but was invariant for propionate. The products of de novo biosynthesis varied only slightly with temperature or cholesterol supplementation, suggesting that de novo biosynthesis is not directly influenced by membrane fluidity. Cerulenin inhibited de novo biosynthesis in a fashion that suggests the presence of two beta-ketoacyl thioester synthetases, which differ in substrate chain length specificity and in susceptibility to inhibition by the antibiotic.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005875738

Download citation: RISBibTeXText

PMID: 914777


Related references

Membrane lipid biosynthesis in Acholeplasma laidlawii B: incorporation of exogenous fatty acids into membrane glyco- and phospholipids by growing cells. Journal of Bacteriology 132(2): 485-496, 1977

Membrane lipid biosynthesis in acholeplasma laidlawii b relationship between fatty acid structure and the positional distribution of esterified fatty acids in phospho lipid and glyco lipids from growing cells. Archives of Biochemistry and Biophysics 182(2): 443-454, 1977

Membrane lipid biosynthesis in Acholeplasma laidlawii b: elongation of medium- and long-chain exogenous fatty acids in growing cells. Journal of Bacteriology 133(1): 66-74, 1978

Membrane lipid regulation in Acholeplasma laidlawii grown with saturated fatty acids. Biosynthesis of a triacylglucolipid forming reversed micelles. Journal of Biological Chemistry 268(22): 16198-16207, 1993

Membrane lipid biosynthesis in Acholeplasma laidlawii B. Investigations into the in vivo regulation of the quantity and hydrocarbon chain lengths of de novo biosynthesized fatty aicds in response to exogenously supplied fatty acids. Archives of Biochemistry and Biophysics 182(2): 455-464, 1977

The biosynthetic incorporation of short-chain linear saturated fatty acids by Acholeplasma laidlawii B may suppress cell growth by perturbing membrane lipid polar headgroup distribution. Biochemistry (American Chemical Society) 41(27): 65-71, 2002

Control of membrane polar lipid composition in Acholeplasma laidlawii a by the extent of saturated fatty acid synthesis. Biochimica et Biophysica Acta 595(2): 189-199, 1980

Membrane lipid metabolism in Acholeplasma laidlawii A EF 22. Influence of cholesterol and temperature shift-down on incorporation of fatty acids and synthesis of membrane lipid species. European Journal of Biochemistry 85(1): 65-76, 1978

Regulation of membrane lipid fluidity in acholeplasma laidlawii b by alteration of de novo fatty acid biosynthetic specificity in the presence of an exogenous fatty acid. Canadian Federation of Biological Societies Proceedings 19: 116, 1976

Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria grown in host cells: is Mycobacterium leprae competent in fatty acid biosynthesis?. Journal of General Microbiology 136(1): 211-217, 1990

Incorporation of short-chain fatty acids by Acholeplasma laidlawii may suppress growth by disruption of membrane lipid polar headgroup distribution. Biophysical Journal 70(2 Part 2): A336, 1996

Biosynthesis of saturated very long chain fatty acids by purified membrane fractions from leek epidermal cells. Archives of Biochemistry and Biophysics 191(1): 146-152, 1978

Calorimetric studies of the thermotropic phase behavior of fatty acid homogeneous acholeplasma laidlawii b cells membranes and membrane lipid dispersion. Biophysical Journalpart 2: 394a, 1986

Biosynthesis and metabolism of saturated fatty acids in the developing and adult brain in relation to the incorporation and turnover of fatty acids in membrane phospholipids. Biochemical Society Transactions 4(2): 234-235, 1976

Fluorine-19 nuclear magnetic resonance studies of lipid fatty acyl chain order and dynamics in Acholeplasma laidlawii B membranes. A physical, biochemical, and biological evaluation of monofluoropalmitic acids as membrane probes. Biochemistry 22(22): 5097-5103, 1983