EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Metabolic physiological and kinetic aspects of the alcoholic fermentation of whey permeate by kluyveromyces fragilis nrrl 665 and kluyveromyces lactis ncyc 571



Metabolic physiological and kinetic aspects of the alcoholic fermentation of whey permeate by kluyveromyces fragilis nrrl 665 and kluyveromyces lactis ncyc 571



Enzyme & Microbial Technology 7(6): 287-294



Optimum growth conditions for the fermentation of non-concentrated whey permeate by K. fragilis NRRL 665 were defined. Use of 3.75 g yeast extract l-1, a growth temperature of 38.degree. C and a pH of 4.0 allowed a maximum productivity of 5.23 g ethanol l-1 h-1 in continuous culture with a yield 91% of theoretical. Complete batch fermentation of permeate with 100 g lactose l-1 was possible with a maximum specific growth rate of 0.276 h-1 without any change in ethanol yield. Fermentation of concentrated permeate resulted in a general decrease of specific substrate consumption rate, demonstrated by the inability to completely convert an initial 90 or 150 g lactose l-1 in continuous culture, even at dilution rates as low as 0.05 and 0.08 h-1, respectively. The decrease could be related to substrate inhibition, to an increase in osmotic pressure caused by lactose and salts and to ethanol inhibition of alcohol and biomass yield. The decrease in specific productivity could be counterbalanced by use of high cell density cultures, obtained by cell recycle of K. fragilis. Fermentation of a non-concentrated permeate at a dilution rate of 1 h-1 resulted in a productivity of 22 g l-1 h-1 at 22 g ethanol l-1. Cell recycle using flocculating K. lactis NCYC 571 was also tested. With this strain a productivity of 9.3 g l-1 h-1 at 45 g product l-1 was attained at a dilution rate of 0.2 h-1, with an initial lactose concentration of 95 g l-1.

Accession: 005881526

Download citation: RISBibTeXText

DOI: 10.1016/0141-0229(85)90088-2

Download PDF Full Text: Metabolic physiological and kinetic aspects of the alcoholic fermentation of whey permeate by kluyveromyces fragilis nrrl 665 and kluyveromyces lactis ncyc 571



Related references

Production of an alcoholic beverage by fermentation of whey permeate with Kluyveromyces fragilis I: Primary metabolism. Journal of the Institute of Brewing 106(6): 367-375, November-December, 2000

Production of an alcoholic beverage by fermentation of whey permeate with Kluyveromyces fragilis II: Aroma composition. Journal of the Institute of Brewing 106(6): 377-382, November-December, 2000

Simulation of process conditions of continuous ethanol fermentation of whey permeate using alginate entrapped Kluyveromyces marxianus NCYC 179 cells in a packed-bed reactor system. Process Biochemistry 23(1): 17-22, 1988

An investigation of ethanol inhibition and other limitations occurring during the fermentation of concentrated whey permeate by kluyveromyces fragilis. Biotechnology Letters 7(7): 521-526, 1985

Effect of aeration rate on the alcoholic fermentation of whey by Kluyveromyces fragilis. Microbiologia 8(1): 14-20, 1992

Effect of the initial concentration of whey on the alcoholic fermentation by Kluyveromyces fragilis. 2006

Alcohol production from whey permeate by immobilized and free cells of Kluyveromyces marxianus NCYC 179. Process Biochemistry 18(4, Supplement): xi, 1983

Production of RNA derivatives by Kluyveromyces fragilis grown on whey Produccion de derivados de RNA mediante Kluyveromyces fragilis inoculada en suero de queso. Food Science and Technology International 3(6): 437-444, 1997

Salted whey utilization. III. Lactose utilization by some yeast species Kluyveromyces lactis, Kluyveromyces fragilis, Candida pseudotropicalis and Torulopsis candida. Egyptian journal of dairy science 11(1): 1-5, 1983

Bioconversion of whey permeate into Kluyveromyces lactis biomass. Egyptian Journal of Dairy Science 20(2): 261-271, 1992

Continuous ethyl acetate production by Kluyveromyces fragilis on whey permeate. Applied microbiology and biotechnology 40(2-3): 201-205, 1993

Ethanol production from concentrated whey permeate using a fed-batch culture of Kluyveromyces fragilis. Biotechnology letters 16(2): 205-210, 1994

Hydrolysis of lactose in whey permeate by immobilized beta-galactosidase from Kluyveromyces fragilis. Journal of Molecular Catalysis B Enzymatic 10(6): 631-637, 1 November, 2000

Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis. Journal of Bacteriology 164(3): 1373-1375, 1985

Interspecific hybrid production between the yeasts kluyveromyces lactis and kluyveromyces fragilis by protoplast fusion. FEMS Microbiology Letters 4(1): 31-34, 1978