Metabolism of 2 4 d in cell suspension cultures of soybean glycine max cultivar mandarin and wheat triticum aestivum cultivar koga ii 2. evidence for incorporation into lignin

Scheel, D.; Sandermann, H.J.

Planta (Heidelberg) 152(3): 253-258


ISSN/ISBN: 0032-0935
Accession: 005882704

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Cell suspension cultures of soybean (G. max L.) and wheat (T. aestivum L.) incorporated 2,4-D into a metabolite fraction which was insoluble in ethanol, water and hot sodium dodecylsulfate. Further treatment with hot dimethylformamide solubilized a material which by the following criteria appeared to consist of 2,4-D derivatives covalently bound to lignin: co-chromatography of radioactivity and of UV-absorbing material upon gel permeation chromatography; spectral similarity with authentic lignins (IR- and UV-spectra, phloroglucinol reaction). 2,4-D appeared to be incorporated as the intact molecule, as shown by comparison of ring- and sidechain-labeled 2,4-D and by detection of monohydroxylated and intact 2,4-D as the major radioactive products of acid hydrolysis. The same compounds were released from the metabolite material which could not be solubilized in dimethylformamide. The incorporation of xenobiotics or their metabolites into lignin, followed by deposition in the cell wall, is suggested as a general pathway for local excretion and detoxification by plant cells.