Metabolism of phenyl alanine and cinnamic acid in tobacco nicotiana tabacum cultivar xanthi cell lines with high and low yields of cinnamoyl putrescines

Berlin, J.; Witte, L.

Journal of Natural Products 45(1): 88-93


ISSN/ISBN: 0163-3864
Accession: 005884860

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The different yields of cinnamoyl putrescines by 2 cell lines of N. tabacum were accounted for by the different activities of enzymes involved in their biosynthesis. The metabolism of the precursors phenylalanine and cinnamic acid were compared in these cell lines. The high producing cell line tolerated 10-2 M L-phenylalanine without any growth inhibition and incorporated 3 times more phenylalanine into cinnamoyl putrescines than the low yielding line. The incorporation of exogenously added phenylalanine resulted in a corresponding increase of cinnamoyl putrescines. When phenylalanine levels were increased from 2 .cntdot. 10-3 to 10-2 M total incorporation was unchanged even in the presence of precursors of the amine moiety. Cinnamic acid was quite toxic to both cell lines and was not directly incorporated into cinnamoyl putrescines. During the 1st hours of feeding, ethyl acetate-soluble metabolites were dominant which were then converted to water-soluble metabolites. Though most of the added cinnamic acid was finally found as cinnamoyl putrescines, the incorporation apparently did not follow the natural biosynthetic pathway.