Methods for the isolation and characterization of constituents of natural products part 21 use of a celite potassium methylate column for rapid preparation of methyl esters from microgram amounts of glycerides

Schwartz, D.P.

Microchemical Journal 22(4): 457-462


ISSN/ISBN: 0026-265X
Accession: 005890481

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In a convenient, rapid procedure, a very small column of potassium methylate-Hyflo Super-Cel is used to convert microgram amounts of glycerides to methyl esters. Transesterification is complete in hydrocarbon but not in chlorinated solvents or in CS2. The methyl esters can be recovered in 92-95% yield if desired. Regardless of the solvent used, the recovered methyl esters are representative of the original fatty acid composition of the glycerides. [The method was used with various synthetic glyceridaes and milkfat, beef tallow and corn, cottonseed, olive, safflower and soybean oils.].