Microtechniques for flow cytometric analysis of aqueous humor lymphocytes

Freeman, W.R.; Char, D.H.; Garavoy, M.R.; Lyon, H.

Journal of Immunological Methods 73(2): 447-455


ISSN/ISBN: 0022-1759
PMID: 6333465
DOI: 10.1016/0022-1759(84)90420-4
Accession: 005900224

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Flow cytometric analysis of monoclonal antibody-labeled lymphocytes is an important tool in understanding the immune response. Microtechniques were developed to analyze lymphocytes in small amounts of ocular fluids. Optimal labeling volumes and concentrations for Leu 2a, Leu 3a, Leu 4 and Leu M3 antibodies were determined. The ability to label and reproducibly analyze cell samples with as few as 1000 cells is demonstrated.