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Milk replacers for preruminant lambs limiting amino acids in 2 soybean protein isolates determined with a changeover design



Milk replacers for preruminant lambs limiting amino acids in 2 soybean protein isolates determined with a changeover design



Australian Journal of Agricultural Research 30(1): 125-134



Preruminant male crossbred lambs, aged about 2 wk at the start of the experiment, were used. Low protein milk replacers (0.10 of total energy as protein) containing isolated soybean protein (ISP-A or ISP-B) as the sole source of protein were supplemented with mixtures of amino acids. In experiment 1a, lambs (6) were each given 6 isonitrogenous diets in a 6 .times. 6 change-over design with 4 day dietary periods. The diets contained ISP-B and were supplemented with methionine alone, or methionine plus lysine, isoleucine, threonine, valine or tryptophan. In experiment 1b, groups of 6 lambs were given diets containing either ISP-A or ISP-B. Each lamb within a group was given 6 diets in a 6 .times. 6 change-over design with 4 day dietary periods. One diet was supplemented with 6 amino acids (Met, Lys, Isol, Thr, Val and Try) while the other 5 diets had 1 amino acid (not Met) omitted. N balances were determined in experiments 1a and 1b on the last 2 days of each dietary period. In experiment 2, groups (2) of 4 lambs were used in a conventional 12 day N balance experiment. One group was given the milk replacer containing ISP-B supplemented with the 6 amino acids used in experiment 1b, and the other group the same basal diet supplemented only with Met, Lys and Thr. The addition of single amino acids in addition to methionine did not improve N balances. Lambs given diets supplemented with Met plus Thr, Val or Try had significantly lower N balances than those given a supplement of Met alone (experiment 1a). Lambs given diets in which Lys or Thr were omitted from the mixture of 6 amino acids had lower N balances than any other lambs (experiment 1b). Lys and Thr were equally 2nd limiting in ISP-A and ISP-B at the 0.10 protein energy concentration. The retention of apparently digested N by lambs given ISP-B supplemented with Met, Lys and Thr (0.669) was significantly higher than that of lambs given ISP-B supplemented with 6 amino acids (0.590; experiment 2). The mean digestibility coefficient of nitrogen in ISP-B (0.842 .+-. 0.009) was significantly higher than that in ISP-A (0.764 .+-. 0.014) and the diets containing ISP-B were more readily accepted by the lambs.

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Accession: 005902502

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