+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria : I. Isolation, localization and allelism of suppressors



Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria : I. Isolation, localization and allelism of suppressors



Current Genetics 9(1): 1-10



A systematic search for suppressors of mutations which cause a deficiency in the splicing of mitochondrial RNA has been undertaken. These splicing mutations were localized in the mRNA-maturase coding sequence of the second intron of the cob-boxgene, i.e. in the box3locus. A total of 953 revertants (mostly spontaneous in origin) were isolated and their genetic nature (nuclear vs. mitochondrial) and phenotype characterized.Most revertants were mitochondrially determined and displayed a wild-type phenotype. A mitochondrial suppressor unlinked with the box3 (-)target mutation was uncovered among the revertants displaying a pseudo-wild phenotype: out of 26 revertants analyzed, derived from 7 different box3(-) mutants only one such suppressor mutation mim3-1 was found. It was localized by rho(-) deletion mapping in the region between the oxi2 and oxi3 gene, within (or in the vicinity) the gene specifying the 15S ribosomal RNA.Nuclear suppressors were isolated from seven different box3 (-)mutants. All were recessive and had a pseudo-wild phenotype. Three such suppressors nam3-1, nam3-2 and nam3-3 were investigated more extensively. Tetrad analysis has shown that they are alleles of the same nuclear locus NAM3 and mitotic analysis has shown that they do not segregate mitotically.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 005905272

Download citation: RISBibTeXText

PMID: 24173504

DOI: 10.1007/bf00396198


Related references

Mitochondrial and nuclear mitoribosomal suppressors that enable misreading of ochre codons in yeast mitochondria : II. Specificity and extent of suppressor action. Current Genetics 9(1): 11-19, 1984

Nuclear omnipotent suppressors of premature termination codons in mitochondrial genes affect the 37s mitoribosomal subunit. Current Genetics 13(2): 129-136, 1988

Nuclear suppressors of mitochondrial chloramphenicol resistance in Baker's yeast: their use for the isolation of novel mutants. Current Genetics 8(2): 121-126, 1984

Nuclear suppressors of the mitochondrial mutation oxi 1 v 25 in saccharomyces cerevisiae i. the phenotypes of some suppressors. Current Genetics 9(6): 427-434, 1985

Identification of transfer RNA suppressors in Escherichia coli. III. Ochre suppressors of lysine tRNA. Journal of Molecular Biology 177(4): 609-625, 1984

Nuclear suppressors of the mitochondrial mutation oxi1-V25 in Saccharomyces cerevisiae. Genetic analysis of the suppressors: absence of complementation between non-allelic mutants. Journal of General Microbiology 132(8): 2087-2097, 1986

The conversion of amber suppressors to ochre suppressors. Proceedings of the National Academy of Sciences of the United States of America 60(3): 1030-1037, 1968

Identification of transfer rna suppressors in escherichia coli 3. ochre suppressors of lysine transfer rna. Journal of Molecular Biology 177(4): 609-626, 1984

A new class of ochre suppressors in the yeast Saccharomyces: mutation in the glutaminyl tRNA gene. Doklady: biological sciences Akademiia nauk SSSR 310(1-6): 61-64, 1990

Yeast uaa suppressors effective in psi positive strains leucine inserting suppressors. Journal of Molecular Biology 132(3): 507-520, 1979

Modifiers of ochre suppressors in saccharomyces cerevisiae that exhibit ochre suppressor dependent amber suppression. Current Genetics 14(4): 345-354, 1988

Yeast uaa suppressors effective in psi plus strains serine inserting suppressors. Journal of Molecular Biology 128(1): 81-100, 1979

Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA. Cell 7(3): 381-390, 1976

Mutagenesis inEscherichia coliV. Attempted interconversion of ochre and amber suppressors and mutational instability due to an ochre suppressor. Molecular Genetics and Genomics 107(4): 351-360, 1970

Mutagenesis in Escherichia coli. V. Attempted interconversion of ochre and amber suppressors and mutational instability due to an ochre suppressor. Molecular and General Genetics 107(4): 351-360, 1970