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Mitochondrial dna variation in cytoplasmic male sterile somatic hybrids of brassica napus



Mitochondrial dna variation in cytoplasmic male sterile somatic hybrids of brassica napus



Plant Physiology and Biochemistry 25(3): 249-258



Mitochondrial (mt) DNA Sal I restriction enzyme analysis of Brassica napus cybrid plants, which combine Brassica napus chloroplasts and a cytoplasmic male sterility trait from Raphanus sativus, revealed that 5 cybrid patterns contain both parental fragments and novel ones. The largest novel fragment, 42 kb in size, previously isolated from a cosmid library was found to be constituted by fragments of both parent mitochondrial DNAs. Hybridization experiments between blots of B. oleracea, B. napus and cybrids mt DNA Sal I patterns and the nick-translated novel bands used as probes indicated that mitochondrial recombination events arising through protoplast fusion involve several different mitochondrial DNA regions and that some events occur at regions which are homologous to regions supposed to play a role in natural recombination. Gene mapping experiments revealed that the 26 Smitochondrial rRNA gene is involved in some mitochondrial events in cybrids. The possibility that mitochondrial ribosomal genes may represent cytoplasmic male sterility factors is suggested from comparison between rRNA mapping and fertility restoration experiments.

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