Modifications of the activities of key enzymes and intracellular levels of cyclic nucleotides, in correlation with the glyogen deposition in a cultured hepatoma cell line

Staedel, C.; Castagna, M.; Beck, J.P.

Cell Differentiation 8(1): 29-38


ISSN/ISBN: 0045-6039
PMID: 222482
Accession: 005913056

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Glycogen accumulation in growing cultures of AMP, whereas cGMP rises early after replating and then decreases simultaneously with the growth rate. These cultured hepatoma cells apparently undergo throughout every passage an induction process involved in glycogen synthesis and storage. Since the original ascites cells growing in vivo (which lack glycogen) and the cultured ZHC cells exhibit similar glycogen synthetase and phosphorylase activities, the resurgence of the glycogenic function in the in vitro cultured cells does not seem related to a change in these 2 enzymes. The high cyclic nucleotide levels in the cultured cells, as compared to those in the ascites cells, offer a possible explanation.