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Molecular size variation of epstein barr virus induced nuclear antigen is determined by the epstein barr viral genome studies on sublines of epstein barr virus negative lymphomas converted with different epstein barr virus substrains and on somatic hybrids



Molecular size variation of epstein barr virus induced nuclear antigen is determined by the epstein barr viral genome studies on sublines of epstein barr virus negative lymphomas converted with different epstein barr virus substrains and on somatic hybrids



Intervirology 22(2): 85-96



The size classes of the Epstien-Barr virus(EBV)-induced nuclear antigen (EBNA) were determined in EBV-carrying sublines of 2 originally EBV-negative Burkitt lymphomas, converted in vitro by 2 different EBV substrains, and in somatic hybrids between 2 EBV-carrying cells or between an EBV-positive and an EBV-negative line. Partially purified EBNA components were detected by Western blotting and subsequent exposure to antiserum/alkaline-phosphatase-coupled protein A complexes. Converted Ramos and BJAB sublines contained the main EBNA components characteristic for the virus donor strain. The EBNA components of the Ramos and BJAB cells converted by P3HR-1 virus were similar to each other and to the components of the P3HR-1 donor cell; the EBNA of the cells converted by B95-8 virus resembled the B95-8 donor line. It was concluded that the size variation of EBNA is determined by the viral genome. The parental lines of the somatic hybrids studied (Raji, P3HR-1, Namalwa and Daudi) contained EBNA components of different MW classes. The higher MW forms ranged from 70 to 80K. Several distinctive lower MW components also were present. The somatic hybrids expressed the main EBNA components of both parental lines. Hybrids between EBV-carrying and EBV-genome-negative lines contained the characteristic EBNA component of the EBV-positive parent. It was concluded that the size variation of EBNA is under strict genetic control which prevails in the hybrids in a codominant fashion.

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