Molecular weight distribution of cellulose in primary cells walls investigations with regenerating protoplasts suspension cultured cells and mesophyll of tobacco nicotiana tabacum cultivar xanthi

Blaschek, H.K.; Semler, U.; Franz, G.

Planta (Heidelberg) 154(6): 550-555


ISSN/ISBN: 0032-0935
Accession: 005920296

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The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized 14C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, and, after fractionation according to differences in solubility in acetone/water, the DP pattern of labeled or unlabeled cellulose nitrate was determined by viscosity measurements. A low (DP < 500) and high DP-fraction (DP > 2500) of cellulose were predominant in the cell walls of protoplasts, suspension-cultured cells, and mesophyll cells. The average DP of the high MW fraction of cellulose in the cell walls of mesophyll was higher (DP .apprx.4000) than in protoplasts or suspension-cultured cells (DP 2500-3000). In all cell walls tested, minor amounts of cellulose molecules with a broad spectrum of a medium DP were present. Pluse-chase experiments with either protoplasts or suspension-cultured cells showed that a large proportion of the low and medium DP-cellulose are a separate class of structural components of the cellulose network. The organization of cellulose in the primary cell wall is discussed.