EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Monoclonal antibodies against human null acute lymphoblastic leukemia cell line nall 1 2. 2 unique antibodies specific for leukemia associated antigens


Monoclonal antibodies against human null acute lymphoblastic leukemia cell line nall 1 2. 2 unique antibodies specific for leukemia associated antigens



Okayama Igakkai Zasshi 96(5-6): 489-502



ISSN/ISBN: 0030-1558

Two monoclonal antibodies (NH-1 and NH-2 ab) were produced by immunizing BALB/c mice with a null-acute lymphoblastic leukemia (null-ALL) cell line, NALL-1, and fusing the mouse spleen cells to mouse myeloma cell line, P3-NS-1/1-Ag4-1. The resulting hybrid cells were screened; and 2 stable monoclonal ab-producing cell lines were isolated by repeated clonings. Reactivities of the ab were analyzed using an indirect membrane immunofluorescence test against cultured cell lines, normal peripheral blood cells, bone marrow cells, lymph node cells, spleen cells, hematopoietic tumor cells and non-hematopoietic tumor cells. NH-1 ab reacted exclusively with monocytes and leukemia cells of null-ALL, acute myelocytic leukemia, acute promyelocytic leukemia, acute monocytic leukemia and chronic myelocytic leukemia in blast crisis (CML-BC). NH-2 ab did with leukemia cells from some cases of null-ALL and CML-BC. Mature granulocytes and lung adenocarcinoma cells also reacted with NH-2 ab. When HL-60 cells were cultured with dimethylsulfoxide, expression of NH-1 reactive antigen (NH-1 ag) on the differentiated HL-60 cells was decreased, but NH-2 ag was not expressed. When NALL-1 cells were cultured with NH-1 or NH-2 ab, NH-2 ag modulated, but NH-1 ag did not. Ig of both abs was IgG and did not exhibit complement-dependent cytotoxic activity. NH-1 ag was apparently a differentiation ag expressed commonly by immature hematopoietic tumor cells and monocytes. NH-2 ag may be a unique common ALL ag which is also expressed on mature granulocytes and lung adenocarcinoma cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005923459

Download citation: RISBibTeXText

Related references

Antibodies to human null acute lymphoblastic leukemia cell line nall 1 1. monoclonal antibodies with specificities for hla ia like and human leukocyte common antigens. Okayama Igakkai Zasshi 96(1-2): 137-148, 1984

Monoclonal antibodies against MHC class Ii antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line. Neoplasma 32(6): 641-648, 1985

Analysis of two new leukemia-associated antigens detected on human T-cell acute lymphoblastic leukemia using monoclonal antibodies. Blood 62(4): 852-865, 1983

Application of a series of monoclonal antibodies against human white cell differentiation antigens and the common acute lymphoblastic leukemia antigen in the characterization of leukemia cells. Archivum Immunologiae et Therapiae Experimentalis 32(1): 43-50, 1984

Studies on antigens of a leukemic null cell line nall 1 2. a leukemia lymphoma associated antigen detected by rabbit anti nall 1 serum. Okayama Igakkai Zasshi 95(7-8): 643-652, 1983

Synovial fluid involvement in null cell acute lymphoblastic leukemia diagnosed with monoclonal antibodies. Journal of Rheumatology 15(3): 500-504, 1988

Human t cell acute lymphoblastic leukemia specific monoclonal antibodies sn 1 and sn 1a determination of the specificity by means of multiple assay procedures. Proceedings of the American Association for Cancer Research Annual Meeting 27: 331, 1986

Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies. Proceedings of the National Academy of Sciences of the United States of America 79(14): 4386-4390, 1982

Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor. Blood 92(11): 4317-4324, 1998

Antibodies TC-12 ("unique") and TH-111 (CD96) characterize T-cell acute lymphoblastic leukemia and a subgroup of acute myeloid leukemia. Experimental Hematology 26(13): 1209-1214, 1998

Human monoclonal antibodies reactive with cell surface antigens on human leukemia cell lines: many antibodies are (auto)antibodies. Hybridoma 9(2): 97-110, 1990

NALL-1, an acute lymphoblastic leukemia cell line with peroxidase activity. In Vitro 20(11): 884-888, 1984

Human myeloid differentiation antigens identified by monoclonal antibodies to the myelomonocytic leukemia cell line RC-2A. Pathology 20(2): 137-146, 1988

Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Research 47(8): 2160-2166, 1987

Demonstration of antibodies binding to autologous and allogeneic leukemic cells in childhood acute lymphoblastic leukemia evidence for a common acute lymphoblastic leukemia antigens. Blut 52(6): 337-344, 1986