+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Monoclonal antibodies directed against protoplasts of soybean glycine max cells generation of hybridomas and characterization of a monoclonal antibody reactive with the cell surface



Monoclonal antibodies directed against protoplasts of soybean glycine max cells generation of hybridomas and characterization of a monoclonal antibody reactive with the cell surface



Planta (Heidelberg) 168(4): 503-511



Splenocytes, derived from mice that had been immunized with protoplasts prepared from suspension cultures of root cells of Glycine max (L.) Merr. (SB-1 cell line), were fused with a murine myeloma cell line. The resulting hybridoma cultures were screened for the production of antibodies directed against the soybean protoplasts and were then cloned. One monoclonal antibody, designated MVS-1, was found to bind to the outer surface of the plasma membrane on the basis of several criteria: (a) agglutination of the protoplasts; (b) binding of fluorescence-labeled immunoglobin on protoplasts yielding a ring staining pattern with prominent intensity at the edges; and (c) saturable binding by protoplasts of 125I-labeled Antibody MVS-1. The antigenic target of Antibody MVS-1, identified by immunoblotting techniques, contained a polypeptide of relative molecular mass (Mr) approx. 400,000 under both reducing and non-reducing conditions. When the antigenic target of Antibody MVS-1 was chormatographed in potassium phosphate buffer, the position of elution corresponded to that of a high-molecular-weight species (Mr 400,000). These results provide the protein characterization required for the analysis of the mobility of Antibody MVS-1 bound to the plasma membrane of SB-1 cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005923616

Download citation: RISBibTeXText


Related references

Monoclonal antibodies directed against protoplasts of soybean cells: Generation of hybridomas and characterization of a monoclonal antibody reactive with the cell surface. Planta 168(4): 503-511, 1986

Monoclonal antibodies directed against protoplasts of soybean cells : Generation of hybridomas and characterization of a monoclonal antibody reactive with the cell surface. Planta 168(4): 503-511, 1986

Monoclonal antibodies directed against protoplasts of soybean cells: analysis of the lateral mobility of plasma membrane-bound antibody MVS-1. Journal of Cell Biology 102(4): 1350-1357, 1986

Generation and characterization of rat monoclonal anti idiotypic antibodies to a human monoclonal antibody reactive with tumor associated antigens. Proceedings of the American Association for Cancer Research Annual Meeting 31: 299, 1990

Generation and characterization of monoclonal antibodies directed against the surface antigens of cervical cancer cells. Hybridoma and Hybridomics 23(2): 121-125, 2004

Production of precursor t cell hybridomas and monoclonal antibodies directed to surface antigens of them. Cell Structure & Function 16(6): 585, 1991

Human monoclonal IgM antibodies from foetal B-cell hybridomas directed against a surface antigen on human tumour cells. Research In Immunology. 143(9): 879-891, 1992

Generation of Monoclonal Antibodies against Plant Cell-Wall Polysaccharides: I. Characterization of a Monoclonal Antibody to a Terminal -(12)-Linked Fucosyl-Containing Epitope. Plant Physiology 104(2): 699-710, 1994

Generation of monoclonal antibodies against plant cell-wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal a-(1- >2)-linked fucosyl-containing epitope. Plant Physiology 104: 9-710, 1994

Generation of monoclonal antibodies against plant cell-wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1-->2)-linked fucosyl-containing epitope. Plant Physiology 104(2): 699-710, 1994

Preparation and characterization of hybridomas secreting monoclonal antibodies directed against the cell adhesion recognition sequence of cadherins. Molecular Biology of the Cell 3(Suppl. ): 215A, 1992

Monoclonal antibodies directed against the microtubule-associated protein tau, hybridomas secreting these antibodies, antigen recognition by these monoclonal antibodies and their applications. Official Gazette of the United States Patent & Trademark Office Patents 1218(3): 2174, 1999

Isolation and characterization of monoclonal antibodies directed against plant plasma membrane and cell wall epitopes identification of a monoclonal antibody that recognizes extensin and analysis of the process of epitope biosynthesis in plant tissues and cell cultures. Journal of Cell Biology 107(1): 163-176, 1988

The generation of hybridomas secreting human monoclonal antibodies reactive with type II collagen. Hybridoma 8(2): 187-197, 1989

Elimination of chemoresistant multiple myeloma clonogenic colony-forming cells by combined treatment with a plasma cell-reactive monoclonal antibody and a P-glycoprotein-reactive monoclonal antibody. Cancer Research 49(17): 4829-4834, 1989