+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Monoclonal antibodies to newcastle disease virus and their use in the detection of antigenic variation

Monoclonal antibodies to newcastle disease virus and their use in the detection of antigenic variation

Chinese Journal of Virology 4(1): 39-44

Twenty-one hybridoma cell lines secreting monoclonal antibodies (McAbs) specific for Newcastle disease virus (NDV) were established by fusions of myeloma cell line SP2/0 with spleen cells from mice immunized with different strains of NDV covering 3 pathotypes. These McAbs were categorized into 3 groups according to their reactivities in HI, HLI and N tests. 27 reference strains and 8 field isolates of NDV covering 3 pathotypes were tested against these McAb and classified into 7 groups according to their distinctive spectra of binding reactivities. Strains or isolates in the same group shared some characteristics of biological and epidemiological importance. Particularly, 2 McAbs, designated as LD2 and LC10-5, reacted only with the widely used vaccine strains B1, La Sota as well as another isolate with undefined characteristics. These McAbs as judged by their promising features may have wide applications in the diagnosis and epidemiological studies of ND as well as in the identification and serotyping of various pathotypes of NDV.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 005924035

Download citation: RISBibTeXText

Related references

Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Archives of Virology 75(4): 243-253, 1983

Relativity between antigenic variation and epidemiologic characters of newcastle disease virus strains detected by monoclonal antibodies. Virologica Sinica 7(4): 449-455, 1992

Antigenic characterization of the internal proteins of Newcastle disease virus by monoclonal antibodies. Virus Research 7(1): 83-92, 1987

Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies. Research in Veterinary Science 86(1): 174-182, 2008

Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies. Archives of Virology 90(1-2): 97-110, 1986

Pathogenicity of antigenic variants of Newcastle disease virus Italian strain selected with monoclonal antibodies. Annales de Recherches Veterinaires. Annals of Veterinary Research 18(4): 371-374, 1987

Antigenic characterization of the nucleocapsid protein of Newcastle disease virus by means of a new panel of monoclonal antibodies. Comparative Immunology, Microbiology and Infectious Diseases 23(3): 209-220, 2000

Selection of unique antigenic variants of Newcastle disease virus with neutralizing monoclonal antibodies and anti-immunoglobulin. Proceedings of the National Academy of Sciences of the United States of America 82(20): 7106-7110, 1985

Complement-fixation test for detection of viral antibodies in chicken serum. I. Standardization of the test for detection of antibodies to Newcastle disease virus and polyoma virus. II. Immunologic response in chickens following experimental infection with Newcastle disease virus. Avian Diseases 16(5): 1011-1018, 1019-1028, 1972

Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies. Avian Pathology 26(2): 399-418, 1997

Characterization of reference strains of newcastle disease virus and newcastle disease virus like isolates by monoclonal antibodies to hemagglutinin neuraminidase subunits. Archives of Virology 85(1-2): 109-122, 1985

Characterization of Newcastle disease virus field strains by means of three panels of monoclonal antibodies: analysis of variation regularities. Immunobiology of viral infections Proceedings 3rd Congress of the European Society for Veterinary Virology Interlaken, Switzerland, 4-7 September 1994: 475-478, 1995

Monoclonal antibodies as functional probes of the hemagglutinin neuraminidase glycoprotein of newcastle disease virus antigenic separation of the hemagglutinating and neuraminidase sites. Journal of Immunology 133(4): 2215-2219, 1984

Functional and neutralization profile of seven overlapping antigenic sites on the HN glycoprotein of Newcastle disease virus: monoclonal antibodies to some sites prevent viral attachment. Virus Research 13(3): 245-261, 1989

The serology of Newcastle disease virus infection. I. The reaction between various sera and the virus. II. The antigenic relationships of Newcastle virus. III. Prevalence of antibody against Newcastle in individuals closely exposed to the virus and in the absence of conjunctivitis. J.Immunol 76: 342-847; 348-351; 352-356, 1956