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Monoclonal antibodies to newcastle disease virus and their use in the detection of antigenic variation



Monoclonal antibodies to newcastle disease virus and their use in the detection of antigenic variation



Chinese Journal of Virology 4(1): 39-44



Twenty-one hybridoma cell lines secreting monoclonal antibodies (McAbs) specific for Newcastle disease virus (NDV) were established by fusions of myeloma cell line SP2/0 with spleen cells from mice immunized with different strains of NDV covering 3 pathotypes. These McAbs were categorized into 3 groups according to their reactivities in HI, HLI and N tests. 27 reference strains and 8 field isolates of NDV covering 3 pathotypes were tested against these McAb and classified into 7 groups according to their distinctive spectra of binding reactivities. Strains or isolates in the same group shared some characteristics of biological and epidemiological importance. Particularly, 2 McAbs, designated as LD2 and LC10-5, reacted only with the widely used vaccine strains B1, La Sota as well as another isolate with undefined characteristics. These McAbs as judged by their promising features may have wide applications in the diagnosis and epidemiological studies of ND as well as in the identification and serotyping of various pathotypes of NDV.

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