+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Morphological and functional analysis of basophils in chronic myelocytic leukemia cml ii. scanning electron microscopic observations of basophils did examination of ige receptors and histamine content



Morphological and functional analysis of basophils in chronic myelocytic leukemia cml ii. scanning electron microscopic observations of basophils did examination of ige receptors and histamine content



Okayama Igakkai Zasshi 99(7-8): 737-750



To clarify the ultrastructural and functional charcteristics of basophils in CML, the author observed the morphological changes of cells by scanning electron microscopy (SEM), and examined the density and redistribution of IgE receptors using an immuno-SEM technique. Histamine content and release of histamine from basophils upon addition of anti-IgE were also measured. Mature basophils, corresponding to types I and II in the basophilogram, had a spherical shape, while immature cells, corresponding to types III and IV, were pear-shaped. Type I, II, III and IV basophils differed in the number of microvilli, more mature basophils having more microvilli than immature basophils. The mean number of bound immunolatex particles, which indicate IgE receptors, was 19.3 .+-. 14.0/basophil in healthy subjects, 42.4 .+-. 30.9 in bronchial asthmatic, and 3.7 .+-. 6.6 in CML patients. The number of IgE receptors in the blastic phase of CML was much lower than in the chronic phase of CML. The histamine content of basophils was 0.5 .+-. 0.3 pg/cell in the blastic phase of CML compared with 1.4 .+-. 0.4 pg/cell in the chronic phase of CML, 1.3 .+-. 0.4 pg/cell in healthy subjects and 1.4 .+-. 0.4 pg/cell in bronchial asthmatic. These data indicate that immature basophils of CML have some morphological and functional defects.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005928995

Download citation: RISBibTeXText


Related references

Morphological and functional analysis of basophils in chronic myelocytic leukemia cml 1. clinical evaluation of basophils with special reference to the basophilogram in the early diagnosis of blastic crisis. Okayama Igakkai Zasshi 99(7-8): 725-736, 1987

Ultrastructural cytochemistry of basophils in chronic myelocytic leukemia. Experimental & Molecular Pathology 30(1): 41-54, 1979

Case of acute myelocytic leukemia (AML) with an increase in bone marrow basophils and morphological anomalies of 3 blood cell lineages. Nihon Naika Gakkai Zasshi. Journal of the Japanese Society of Internal Medicine 85(10): 1768-1770, 1996

Surface membrane alterations in guinea pig basophils undergoing anaphylactic degranulation. A scanning electron microscopic study. Laboratory Investigation; a Journal of Technical Methods and Pathology 45(1): 58-66, 1981

Histamine release from the basophils of control and asthmatic subjects and a comparison of gene expression between releaser. and nonreleaser basophils. Journal of Immunology 178(7): 4584-4594, 2007

Release of histamine from human basophils neopl chronic myeloid leukemia enz peroxidase. Blood: 722-736, 1967

Studies on the dynamics of basophils. 4. Basophils and histamine. Japanese Journal of Medical Progress 49: 265-273, 1962

Studies on supravital observation of basophils in bronchial asthma in immunological reaction part 2 morphological changes of basophils upon addition of anti immuno globulin. Okayama Igakkai Zasshi 87(11-12): 963-971, 1975

Platelet satellitism to basophils in a patient with chronic myelocytic leukaemia. Blut 45(5): 347-350, 1982

Functional Analysis of Histamine Release from Basophils and Mast Cells in Subjects with the Ile-181Leu Variant of FcRi-. Clinical Science 93(3): 279-286, 1997

Functional analysis of histamine release from basophils and mast cells in subjects with the Ile-181-->Leu variant of Fc epsilon RI-beta. Clinical Science 93(3): 279-286, 1997

Biochemical and functional characterization of proteoglycans isolated from basophils of patients with chronic myelogenous leukemia. Journal of Immunology 132(4): 1943-1950, 1984

Functional analysis of histamine release from basophils and mast cells in subjects with the Ile-181 fwdarw Leu variant of Fc-epsilon-RI-beta. Clinical Science 93(3): 279-286, 1997

Histamine release reaction of basophils in chronic granulocytic leukemia induction by heterologous anti immuno globulin e concanavalin a and phyto hem agglutinin effect of heavy water. Journal of Clinical Pathology 31(8): 749-752, 1978

An experimental carp x tench hybrid karyological analysis and scanning electron microscopic morphological observations. Bollettino di Zoologia 50(3-4): 159-172, 1983