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Morphological changes of rat dorsal root ganglion cells in the process forming period ii. time sequential observation of dorsal root ganglion cells in culture



Morphological changes of rat dorsal root ganglion cells in the process forming period ii. time sequential observation of dorsal root ganglion cells in culture



Sapporo Medical Journal 55(1): 1-10



Rat dorsal root ganglion cells on day 14 of gestation were cultured under the condition that led to a good cell differentiation. The incidence of appearance of pseudounipolar cell was then estimated time-sequentially by silver impregnated preparations. Further, fine structures of dorsal root ganglion cells, especially their proximal portions of processes, were observed with a transmission electron microscope. 1. In silver impregnated preparations, bipolar cells occupy 76% of all ganglion cells at the 6th day of culture, while unipolar cells increase to about 50% at the 8th to 16th day of culture. Unipolar cells increase to about 80% at the 22nd day of culture. 2. Cultured dorsal root ganglion cells at successive developmental stages were observed with a transmission electron microscope. By observing the morphological transformation from bipolar cells to pseudounipolar cells, it is confirmed that the unipolar stem process derives from the elongated cytoplasm of the cell body. No evidence is obtained that the two processes of bipolar cells fuse together to make a stem process.

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