Morphological differentiation and occurrence of thiophenes in leaf callus cultures from tagetes species relation to the growth medium of the plants
Ketel, D.H.
Physiologia Plantarum 66(3): 392-396
1986
ISSN/ISBN: 0031-9317 DOI: 10.1111/j.1399-3054.1986.tb05940.x
Accession: 005930666
The morphology, and the number and concentration of thiophene-like compounds were studied in leaves, roots and calli of Tagetes species grown with different nutrient solutions. The type of nutrient solution exerted no effect on the number of thiophene-like compounds, but altered the type of morphological differentiation and thiophene content of calli. Calli of T. minula L. showed little differentiation and resulted in suspensible callus after two passages. Calli of T. erecta L. cv. Rose d'Inde differentiated rapidly and turned dark brown after one passage. The morphology of calli from T. patula L. cv. Nana furia was intermediate. Tertiary callus of T. patula contained more thiophene-like compounds and higher concentrations than did the corresponding calli of T. minuta. The content of thiophene-like compounds decreased after various callus passages, but the relative decrease varied between species.
