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Morphology and morphometrics of pratylenchus brachyurus and pratylenchus zeae 1. effect of fixative and processing



Morphology and morphometrics of pratylenchus brachyurus and pratylenchus zeae 1. effect of fixative and processing



Indian Journal of Nematology 13(2): 141-154



No one fixative out of F. 4%, F.P. 4: 1, F.A. 4: 1, F.A. 4: 10, T.A.F. and F.A.A. gave the best definition of all morphological features examined in P. brachyurus (Godfrey, 1929) Filipjev and Schuurmans Stekhoven, 1941 and P. zeae Graham, 1951, [plant nematodes] but results with F. 4% and F.P. 4: 1 were most satisfactory. Nematodes stored in plain fixative deteriorated less than those stored in glycerol following processing by Seinhorst's technique. Specimens fixed in T.A.F. and stored in glycerol became transparent but those fixed in F.P. 4: 1 before processing retained their features best. All fixatives caused changes in dimensions but F. 4% and F.P. 4: 1 affected them least. Seinhorst's technique caused more shrinkage but preserved morphological features better than Baker's technique.

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Accession: 005932728

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