Section 6
Chapter 5,944

Multiple hemopoietic lineages are found after stimulation of mouse bone marrow precursor cells with interleukin 3

Prystowsky, M.B.; Otten, G.; Naujokas, M.F.; Vardiman, J.; Ihle, J.N.; Goldwasser, E.; Fitch, F.W.

American Journal of Pathology 117(2): 171-179


ISSN/ISBN: 0002-9440
PMID: 6437231
Accession: 005943144

When the murine T-lymphocyte clone L2 is stimulated with concanavalin A, it secretes at least two distinct factors that affect hemopoietic precursor cells, interleukin 3 (IL3) and granulocyte/macrophage colony-stimulating factor (GM-CSF). IL3 accounts for approximately 10% of the colony-stimulating activity in L2-cell-conditioned medium. The IL3 secreted by L2 cells is similar antigenically to the IL3 secreted by WEHI-3 cells. Like the IL3 from WEHI-3 cells, IL3 secreted by L2 cells does not bind to DEAE Sephacel and can be separated from the L2-cell GM-CSF, which does bind to DEAE. By assessment of the functional, morphologic, surface phenotypic, and cytochemical characteristics of bone marrow cells 6 days after stimulation with IL3 in liquid culture, four hemopoietic lineages were found, including macrophage, neutrophilic granulocyte, megakaryocyte, and basophil/mast cell. In addition, when bone marrow cells were stimulated with IL3 in semisolid medium, several types of colonies were found, including mixed colonies containing macrophage, megakaryocyte, and granulocyte lineages.

PDF emailed within 1 workday: $29.90