Section 6
Chapter 5,945

Multiplication of a densonucleosis virus in the silkworm bombyx mori reared at high temperature

Watanabe, H.; Maeda, S.

Japanese Journal of Applied Entomology and Zoology 23(3): 151-155


ISSN/ISBN: 0021-4914
Accession: 005944782

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Effect of high temperature on multiplication of a densonucleosis virus (DNV) in B. mori was studied using the single radial immunodiffusion method for the virus titration. Multiplication of DNV was greatly reduced when the infected larvae reared at 25-28.degree. C were transferred to an environment at 37.degree. C. Autoradiographic results revealed that synthesis of both viral DNA and protein was greatly reduced in the infected larvae maintained at at 37.degree. C. Fluorescent antibody studies confirmed that synthesis of DNV-antigen in the larva was inhibited at 37.degree. C. Results indicated that high temperature (37.degree. C) may reduce activity of enzymes concerned with viral DNA and protein syntheses.

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