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Chapter 5,946

NusA protein of Escherichia coli is an efficient transcription termination factor for certain terminator sites

Schmidt, M.C.; Chamberlin, M.J.

Journal of Molecular Biology 195(4): 809-818

1987


ISSN/ISBN: 0022-2836
PMID: 2821282
DOI: 10.1016/0022-2836(87)90486-4
Accession: 005945942

We have studied the factors that affect transcription termination in vitro at the tR2 terminator of bacteriophage lambda and at the T1 terminator of the Escherichia coli rrnB operon. Termination efficiency at both of these sites is enhanced by the E. coli nusA protein, giving final efficiencies of termination in vitro comparable to those estimated in vivo. Transcripts terminated in the presence of nusA protein are all released from the RNA polymerase complex, indicating that a complete termination reaction is involved, rather than simply induction of a long pause at the terminator. The termination factor activity of the nusA protein does not depend on the presence of rho protein and is not detectably enhanced by that factor. Thus, the nusA protein appears to play a pleiotropic role in E. coli transcription, serving as an antitermination factor, RNA polymerase subunit and true termination factor for some terminator sites.

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