Mutation of the cyanobacterium anacystis nidulans synechococcus pcc 6301 improved conditions for the isolation of auxotrophs
Herdman, M.; Delaney, S.F.; Carr, N.G.
Archives of Microbiology 124(2-3): 177-184
ISSN/ISBN: 0302-8933 DOI: 10.1007/bf00427724
Previous attempts to isolate auxotrophic mutants of A. nidulans produced only a limited range of phenotypes. The frequency of recovery of auxotrophic mutants was quantified following different mutagenic and selective treatments, and their yield improved by using a complete medium, additional mutagens, multiple cycles of penicillin enrichment and altered pre-enrichment starvation conditions.