Myocardial energy metabolism in congestive and hypertrophic states of cardiomyopathy in hamsters with elastase-induced emphysema

Morimoto, Y.

Nihon Kyobu Shikkan Gakkai Zasshi 20(12): 1221-1229


ISSN/ISBN: 0301-1542
PMID: 6926435
Accession: 005952205

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To examine the kinetics of myocardial energy metabolism and cardiac function in cor pulmonale, comparative studies of the myocardial energy transport system in humans and hamsters with elastase-induced emphysema were made. Effects of digoxin and furosemide on the cardiac metabolism were also pursued. Emphysema in hamsters was induced by 24-U elasterase injections into the trachea. The heart was followed-up more than 1 yr, both histologically and enzymologically. Pyruvate kinase, a key enzyme of glycolysis, was measured by the method of Bucher. The main enzymes of the electron transport system (succinic-cytochrome C reductase, NADH-cytochrome C reductase and cytochrome C oxidase) were measured by the modified method of Tsoo, using a multiconvertible spectrophotometer. The P/O [phosphate/oxygen] ratios were measured polarographically at C with an O2 electrode. In the hypertrophic phase, the activities of pyruvate kinase, NADH-cytochrome C reductase, succinic-cytochrome C reductase and cytochrome C oxidase were increased in the right ventricle. Activities of these enzymes in the left and right ventricular myocardia were decreased in teh congestive phase. The P/O ratio was also decreased only in the congestive phase in the ventricular myocardium. Activities of the human myocardial enzymes were increased in the hypertrophic phase and decreased in the congestive phase. Digoxin and furosemide appeared to exert a small stimulatory effect only in the congestive phase.