+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Natural killer cell recognition of target cells expressing different antigens of vesicular stomatitis virus

Natural killer cell recognition of target cells expressing different antigens of vesicular stomatitis virus

Proceedings of the National Academy of Sciences of the United States of America 82(8): 2456-2459

Natural killer (NK) cells have the capability of lysing virus-infected, transformed and embryonal cells, yet the nature of the target structure(s) recognized remains unclear. The availability of well-characterized temperature-sensitive (ts) mutants of vesicular stomatitis virus, defective in expression of individual viral-encoded polypeptides at the nonpermissive temperature (39.degree. C), offered an approach to elucidating NK-cell recognition of virus-infected cells. Target cells were infected with ts mutants in 3 functions: the viral surface glycoprotein (G protein; ts 045); the matrix (M) protein (ts G31, ts G33), and the polymerase (ts G11). Cells infected with wild-type virus and all ts mutants at the permissive temperature (31.degree. C) were killed by murine spleen cells. Similar to results on cytotoxic T lymphocytes, target cells infected by ts 045 defective in expression of G protein at 39.degree. C were not killed by NK cells. Cells infected at 39.degree. C with M-protein mutants also were not killed, although G protein was expressed at the cell surface. Target binding studies indicated that conjugates were not formed by cells infected with the ts mutants at the nonpermissive temperature. That expression of G protein was not sufficient for NK cell-mediated cytotoxicity was established in experiments in which a plasmid (pSVGL) containing the gene for vesicular stomatitis virus G protein was transfected into COS cells. Although G antigen was expressed on the plasma membrane, the cells were not lysed. These results suggest either that recognition of virus-infected cells depends on an appropriate conformation imparted to the viral G protein by association with the M protein or that NK cells can recognize alterations in the structure of the cell membrane induced by insertion of viral M and G molecules.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005959007

Download citation: RISBibTeXText

PMID: 2986117

DOI: 10.2307/25118

Related references

Target antigens for H-2-restricted vesicular stomatitis virus-specific cytotoxic T cells. Journal of Immunology 121(2): 744-748, 1978

Elicitation of natural killer cells in beige mice by infection with vesicular stomatitis virus. Infection and Immunity 32(1): 204-210, 1981

The involvement of Lyt-2 antigens in the lysis of virus-infected target cells by antigen specific anti-vesicular stomatitis virus cytotoxic T-lymphocytes. Immunology Letters 3(3): 179-181, 1981

Persistence of vesicular stomatitis virus in cloned interleukin-2-dependent natural killer cell lines. Journal of Virology 60(2): 539-547, 1986

Studies on the mechanism of natural killer cell mediated cyto toxicity 1. release of cyto toxic factors specific for natural killer sensitive target cells during co culture of natural killer effector cells with natural killer target cells. Journal of Immunology 129(1): 433-439, 1982

Evolution of vesicular stomatitis virus in athymic nude mice: mutations associated with natural killer cell selection. Journal of General Virology 67: 441-451, 1986

Mono clonal antibodies specific for vesicular stomatitis virus block vesicular stomatitis virus specific t cell target cell conjugation. Federation Proceedings 40(3 PART 2): 1149, 1981

Recognition of virus-infected cells by natural killer cell clones is controlled by polymorphic target cell elements. Journal of Experimental Medicine 178(3): 961-969, 1993

Limitation of vesicular stomatitis virus infection by the host immune response to vesicular stomatitis virus associated cellular antigens. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT E43, 1982

Trojan horse lymphocytes: a vesicular stomatitis virus-specific T-cell clone lyses target cells by carrying virus. Journal of Virology 63(10): 4157-4164, 1989

H-2L-restricted recognition of viral antigens In the H-2d haplotype, anti-vesicular stomatitis virus cytotoxic T cells are restricted solely by H-2L. Journal of Experimental Medicine 156(3): 778-790, 1982

Protection against lethal vaccinia virus challenge by using an attenuated matrix protein mutant vesicular stomatitis virus vaccine vector expressing poxvirus antigens. Journal of Virology 84(7): 3552-3561, 2010

Recombinant replication-restricted vesicular stomatitis virus expressing an IL-12 fusion protein potentiates immune responses to listerial antigens. FASEB Journal 16(4): A312, March 20, 2002

In vitro elicitation of beige and c 57bl 6 natural killer activity by uv inactivated vesicular stomatitis virus. Federation Proceedings 41(4): ABSTRACT 4025, 1982

Replication-competent or attenuated, nonpropagating vesicular stomatitis viruses expressing respiratory syncytial virus (RSV) antigens protect mice against RSV challenge. Journal of Virology 75(22): 11079-11087, 2001