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Natural killing and antibody dependent cellular cyto toxicity in specific pathogen free miniature swine and germ free piglets 1. comparison of natural killing and antibody dependent cellular cyto toxicity



Natural killing and antibody dependent cellular cyto toxicity in specific pathogen free miniature swine and germ free piglets 1. comparison of natural killing and antibody dependent cellular cyto toxicity



Journal of Immunology 125(2): 755-762



Tissue distribution of natural killer (NK) cells and killer (K) cells for antibody-dependent cellular cytotoxicity (ADCC), in vivo and in vitro arming experiments, the effects of Staphylococci protein A and differential susceptibility to pronase treatment of effector cells for NK and ADCC were investigated to determine whether the effector cells for NK and ADCC are the same or distinct subpopulations. NK and ADCC activities were tested by a short-term (2 1/2 to 4 h) 51Cr-release assay with 51Cr-labeled human myeloid [leukemia] K562 cells and TNP[trinitrophenyl]-conjugated human [neoplastic] B cell SB cells used as target cells for NK and ADCC, respectively. In adult specific pathogen-free (SPF) miniature swine, the effector cells for NK were present only in peripheral blood and not in spleen, thymus, mesenteric lymph nodes, bone marrow or tonsil. The K cells for ADCC were present in spleen and bone marrow and in peripheral blood. There was no detectable ADCC activity among cells from thymus, mesenteric lymph nodes or tonsil. In germfree, colostrum-deprived, immunologically virgin piglets, no NK activity was detected in any tissue examined including peripheral blood, spleen, thymus, lymph nodes, bone marrow and liver. K cells for ADCC were present in bone marrow, liver and peripheral blood. Cells from colstrum-fed newborn piglets exposed to maternal natural antibodies did not acquire NK activity; ADCC activity remained unchanged, indicating that NK activity is not simply a function of arming with natural antibodies in vivo. In vitro incubation of immunologically virgin piglet cells with sera from NK-positive animals or with culture fluid of NK-positive cells did not arm NK-negative cells to become NK-positive cells. Protein A-bearing Staphyloccoci inhibited ADCC but not NK activity. NK activity was completely abrogated by 0.1% pronase treatment; the ADCC activity was increased by pronase treatment. When the pronase-treated cells were incubated in autologous serum, there was no recovery of NK activity. In vivo arming with natural antibodies or in vitro-secreted antibodies apparently are not involved in NK activity, which support the presence of NK independent of antibody. That K cells for ADCC develop early in ontogeny before the development of the effector cells for NK, that NK cells and K cells in adults have different tissue distributions, and that NK cells are not K cells armed with natural antibodies, suggest that the effector cells for NK and ADCC may be distinct subpopulations.

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Accession: 005959119

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