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Natural regulator of phenolase activity in grapevine



Natural regulator of phenolase activity in grapevine



Prikladnaya Biokhimiya i Mikrobiologiya 18(2): 266-272



The regulator of phenolase activity, i.e., leucoanthocyanidin, was purified from leaves and stems of grapevine. The regulator acted as activator of monophenol oxidase from grapevine leaves and as inhibitor of o-diphenol oxidase from the same source. The kinetic analysis of the reaction showed that the regulator-phenolase binding increased monophenol oxidase and decreased o-diphenol oxidase activity by 2-3 times. The regulator shifted pH optimum of monophenol oxidase toward the alkaline zone, and that of o-diphenol oxidase (MW = 30,000-35,000 with pyrogallol as substrate) toward the acidic zone.

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