Nerve growth factor induced neurite outgrowth of rat pheo chromo cytoma pc 12 cells dependence on extracellular magnesium ii and calcium ii

Koike, T.

Brain Research 289(1-2): 293-304

1983


ISSN/ISBN: 0006-8993
Accession: 005965144

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Abstract
Dependence of neurite outgrowth on extracellular Mg2+ and Ca2+ was studied in nerve growth factor-responsive pheochromocytoma PC12 cells under assay conditions in which neurite formation was independent of both RNA synthesis and protein synthesis. NGFor 0.5 mM LaCl3. Other divalent cations so far tested proved to be negative, suggesting that this phenomenon appears to be specific to Mg2+. Quantitative analysis revealed that the length and thickness of neurites formed were controlled by the presence of extracellular Ca2+. Thus, neurites formed at lower concentrations of Ca2+ in the presence of 1.0 mM Mg2+ and NGF were found to be thinner and longer than those formed at higher concentrations of Ca2+, suggesting that Ca2+ and Mg2+ have separate regulatory functions in the formation of neurites of PC 12 cells.