+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Novel composition of mitochondrial genomes in petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants



Novel composition of mitochondrial genomes in petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants



Molecular & General Genetics 190(3): 459-467



The mitochondrial genomes of petunia somatic hybrid plants, which were derived from the fusion of male fertile P. hybrida protoplasts with cytoplasmic male sterile P. parodii protoplasts, were analyzed by endonuclease restriction and Southern blot hybridization analyses. Sterile and fertile somatic hybrids were studied to address 2 main questions. First, is there any correlation between the mitochondrial DNA restriction banding patterns of the sterile and fertile parents and the banding patterns of the respective sterile and fertile somatic hybrids? Second, does the structure of somatic hybrid mitochondrial genomes differ from the parental mitochondrial genomes? No clear sterile-specific correlation was identified between the mitochondrial DNA restriction patterns of cytoplasmic male sterile somatic hybrids and those of the male sterile parent. No clear relationship was found between the mitochondrial DNA restriction patterns of male fertile somatic hybrids and those of the male fertile parent. In view of this finding and the evidence that cytoplasmic male sterility and fertility segregate in somatic hybrids of petunia (Izhar et al. 1983) the nature of cytoplasmic male sterility in petunia differs significantly from cytoplasmic male sterility in tobacco (Gerstel 1980). Restriction fragment patterns show that the somatic hybrid mitochondrial genomes differ from each other and from both parents. Somatic hybrid mitochondrial genomes consist of DNA fragments derived from both parents in novel combinations. Hybridization data revealed the fates of parental restriction fragments in the somatic hybrids. Parental fragments may be present or absent in all somatic hybrids analyzed, or they may be present in some somatic hybrids and absent in others. These data are consistent with 2 non-exclusive possibilities. Separate DNA molecules might assort following protoplast fusion or intermolecular recombination might occur following protoplast fusion.

(PDF emailed within 0-6 h: $19.90)

Accession: 006001812

Download citation: RISBibTeXText

DOI: 10.1007/bf00331077


Related references

Somatic hybridization in petunia 2. heteroplasmic state in somatic hybrids followed by cytoplasmic segregation into male sterile and male fertile lines. Theoretical and Applied Genetics 57(6): 241-245, 1980

Somatic hybridization in Petunia : Part 2: heteroplasmic state in somatic hybrids followed by cytoplasmic segregation into male sterile and male fertile lines. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 57(6): 241-245, 1980

Mitochondrial Biogenesis || Sequence and Expression of a Fused Mitochondrial Gene, Associated with Petunia Cytoplasmic Male Sterility, Compared with Normal Mitochondrial Genes in Fertile and Sterile Plants. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 319(1193): 199-208, 1988

Sequence and Expression of a Fused Mitochondrial Gene, Associated with Petunia Cytoplasmic Male Sterility, Compared with Normal Mitochondrial Genes in Fertile and Sterile Plants. Philosophical Transactions Biological Sciences 319(1193): 199-208, 1988

Sequence of expression of a fused mitochondrial gene associated with petunia cytoplasmic male sterility compared with normal mitochondrial genes in fertile and sterile plants. Philosophical Transactions of the Royal Society of London B Biological Sciences 319(1193): 199-208, 1988

Analysis of mitochondrial and chloroplast DNA from fertile and cytoplasmic male-sterile Petunia hybrida. Induced variability in plant breeding: Internat Symp of the Sect Mutation and Polyploidy of the European Assoc for Research on Plant Breeding Wageningen Aug 31 Sept 4-1981 Chairman C Broertjes, 1982

Anther and pollen development in male fertile intermediate wheatgrass and in cytoplasmic male sterile plants derived from wheat X wheatgrass hybrids. Dissertation Abstracts International, B 37(11): 5518B, 1977

Localization of mitochondrial proteins in tissue prints of cytoplasmic male sterile cm and fertile petunia lines. Journal of Cell Biology 115(3 PART 2): 300A, 1991

Organizational differences between cytoplasmic male sterile and male fertile Brassica mitochondrial genomes are confined to a single transposed locus. Nucleic Acids Research 21(8): 1903-1909, 1993

Mitochondrial gene organization and expression in petunia male fertile and sterile plants. Journal of heredity 90(3): 362-368, 1999

Differential Mitochondrial Electron Transport through the Cyanide-Sensitive and Cyanide-Insensitive Pathways in Isonuclear Lines of Cytoplasmic Male Sterile, Male Fertile, and Restored Petunia. Plant Physiology 93(4): 1634-1640, 1990

Cytoplasmic male sterility in hybrids of sterile wild beet (Beta vulgaris ssp. maritima) and O-type fertile sugar beet (Beta vulgaris L.): molecular analysis of mitochondrial and nuclear genomes. Molecular Breeding 11(2): 137-148, 2003

Cytoplasmic male sterility in Petunia hybrida: factors affecting mitochondrial ATP export in normal and cytoplasmically male sterile plants. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 76(2): 305-310, 1988

Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the texas male sterile cytoplasm. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 62(3): 213-217, 1982

Mitochondrial dna analyses of fertile and sterile maize zea mays plants derived from tissue culture with the texas male sterile cytoplasm. Theoretical & Applied Genetics 62(3): 213-218, 1982