Nucleotide sequence of the promoter region of the gene cluster for proton-translocating ATPase from Escherichia coli and identification of the active promotor

Kanazawa, H.; Mabuchi, K.; Futai, M.

Biochemical and Biophysical Research Communications 107(2): 568-575

1982


ISSN/ISBN: 0006-291X
PMID: 6215041
Accession: 006005902

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Abstract
A 505-nucleotide long DNA sequence of a part of the gene cluster for the proton-translocating ATPase (pap operon) of E. coli was determined. In the sequence determined upstream of the gene for 14K protein, 2 promoter sequences with reverse directions to each other were found. One of them was identified as the active promoter for the operon by in vitro transcription and the DNase I footprinting technique. The other was located upstream of the pap operon promoter and identified as an active promoter of a gene coding for a small RNA of unknown function adjacent to the operon.