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Oligo nucleotides and nucleotide peptides part 23 application of t 4 rna ligase for oligo nucleotide coupling



Oligo nucleotides and nucleotide peptides part 23 application of t 4 rna ligase for oligo nucleotide coupling



Bioorganicheskaya Khimiya 2(7): 898-902



Bacteriophage T4 RNA ligase catalyzes covalent coupling of tRNA with a synthetic donor oligoribonucleotide p(A)4Cp. The effects of tRNA concentration as well as the reaction kinetics were studied. tRNA and (A)4C in equimolar ratio react readily with p(A)4Cp. Phosphate blocking of the 3'-hydroxy group in p(A)4Cp prevents the occurrence of donor-donor reactions.

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