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On the histochemical application of alcian green






Acta Histochemica 78(4): 165-172

On the histochemical application of alcian green

With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the glycoproteins, and after an oxidation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.

Accession: 006027622

DOI: 10.1016/S0065-1281(86)80049-6

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Related references

Krutsay, M., 1986: The histochemical use of alcian green. With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and...

Krutsay, M., 1984: Use of alcian green in morphological and histochemical problems. With hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated mucopolysaccharides (acid glycosaminoglycans) can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pret...

Wagner, B.M.; Shapiro, S.H., 1957: Application of alcian blue as a histochemical method. Alcian blue was shown by a variety of histochemical studies to be of value in the localization of acid mucopolysaccharides. The present study utilizes this dye at an acid pH in conjunction with the chromotrope 2R dye. Studies of isolated plasma pr...

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Rizzoli, C., 1955: Histochemical bases for the staining of mucopolysaccharides in tissues with alcian blue 8 G.N. Bollettino Della Societa Italiana di Biologia Sperimentale 31(5): 422-425