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On the histochemical application of alcian green



On the histochemical application of alcian green



Acta Histochemica 78(4): 165-172



With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the glycoproteins, and after an oxidation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.

Accession: 006027622

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DOI: 10.1016/S0065-1281(86)80049-6

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