EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Opiate receptors in the brain of the rat optimum conditions for its determination



Opiate receptors in the brain of the rat optimum conditions for its determination



Eksperimentalna Meditsina i Morfologiya 27(1): 9-13



Two types of opiate receptors were investigated in the brain of the rat-delta for endogenous opiates with ligand D-ala-Enkephalin and mju for exogenous opiates with ligand Naloxon. Inhibitors of peptidases were used in the receptor analysis and the dependence of the amount of membrane protein were examined. The results were computerized by an adequate programme. The results for delta receptors were 34,08.+-.0,33 femtomol per mg of protein and Kd=0.65 nM, but tor mju receptors; 62,118.62 .+-. 14.37 femtomol per mg of protein and Kd=1.39 nM. It was established that the binding of the ligand was optimal at concentration of membrane protein around 200 .mu.g in a sample.

(PDF 0-2 workdays service: $29.90)

Accession: 006036626

Download citation: RISBibTeXText



Related references

Opiate receptors in rat brain. Optimization of conditions for their determination. Eksperimentalna Meditsina i Morfologiia 27(1): 9-13, 1988

Evidence for distinct subcellular sites of opiate receptors. Demonstration of opiate receptors in smooth microsomal fractions isolated from rat brain. Journal of Biological Chemistry 256(19): 10017-10023, 1981

Changes in the properties of opiate receptors in rat brain under conditions of tolerance to morphine. Biokhimiya 51(8): 1334-1340, 1986

Naloxazone a novel opiate antagonist irreversible blockade of rat brain opiate receptors in vitro. Cellular & Molecular Neurobiology 2(2): 93-104, 1982

Multiple opiate receptors of brain and spinal cord in opiate addiction. General Pharmacology 22(5): 767-772, 1991

Cyclofoxy a fluorinated opiate receptor ligand highly selective labeling of brain opiate receptors in vivo. Society for Neuroscience Abstracts 11(1): 581, 1985

Mu opiate receptors and delta opiate receptors correlation with high and low affinity opiate binding sites. European Journal Of Pharmacology3: 323-324, 1980

Differential specificity of mu opiate delta opiate and epsilon opiate agents towards opiate receptors within the septum of morphine pre treated cats a behavioral analysis. Neuroscience Letters (SUPPL 7): S268, 1981

Dynorphins similar relative potencies on mu opiate delta opiate and kappa opiate receptors. European Journal of Pharmacology 76(4): 467-468, 1981

Multiple opiate receptors mediation of enkephalin beta endorphin and mu opiate and kappa opiate analgesia by the same sub population of binding sites. Neurology 30(4): 373, 1980

Selective development of tolerance on mu opiate receptors and delta opiate receptors in the central nervous system. Naunyn-Schmiedeberg's Archives of Pharmacology 313(SUPPL): R27, 1980

Auto radiographic localization of opiate receptors in rat brain part 2 the brain stem. Brain Research 129(1): 1-12, 1977

Autoradiographic localization of opiate receptors in rat brain. II. The brain stem. Brain Research 129(1): 1-12, 1977

Evidence for an involvement of mu opiate but not delta opiate or kappa opiate receptors in sympathetically and parasympathetically mediated cardio vascular responses to opiates upon anterior hypothalamic injection. Life Sciences-13: 1279-1282, 1982

Opiate receptors of the brain. Fiziologicheskii Zhurnal 33(2): 98-108, 1987